Vascular embolization of radiopaque hydrogel microfiber using ultra-minimally invasive technique for stage-adjustable renal failure model
Vascular embolization of radiopaque hydrogel microfiber using ultra-minimally invasive technique for stage-adjustable renal failure model
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DOI：
10.1016/j.matdes.2022.110802
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发表时间：
2022-06-06
影响因子：
8.4
通讯作者：
Onoe，Hiroaki
中科院分区：
文献类型：
--
作者：
Takakura，Naoki;Ohta，Hiroki;Onoe，Hiroaki
动物病理模型在阐明病理、评价药物疗效、研究治疗方法等方面发挥着重要作用。采用不透射线的水凝胶微纤维栓塞肾动脉，建立大鼠肾衰模型。采用双同轴层流微流控装置，制备了含有氧化锆颗粒的海藻酸钡凝胶微纤维。由于氧化锆颗粒不透射线，因此可以在x射线成像下实时确定微纤维位置的同时输送和栓塞微纤维。研究了微纤维通过导管进入大鼠肾动脉制造肾衰竭模型的方法。当导管输送时，通过x射线成像可见不透射线的微纤维。此外，微纤维在分娩后2周内保持稳定。此外，通过调节微纤维的数量，建立了两种不同的大鼠重度和轻度肾功能衰竭模型。建议使用不透射线的水凝胶微纤维来创建栓塞模型，有望成为控制症状程度和阐明病理的有效方法。
Animal pathology models play an important role in elucidating pathology, evaluating drug efficacy, and studying therapeutic. This paper presents the creation of rats’ renal failure model by embolizing the renal artery using radiopaque hydrogel microfibers. By using a dual coaxial laminar flow microfluidic device, barium alginate gel microfibers containing zirconia particles were fabricated. Since the zirconia particles are radiopaque, the microfiber can be delivered and embolized while confirming the position of the microfiber in real-time under X-ray imaging. The delivery of microfibers through a catheter into the renal artery of rats to create a renal failure model was tested. The radiopaque microfibers were visualized by X-ray imaging when delivered by a catheter. Furthermore, the microfibers remained stable for 2 weeks after delivery. In addition, by adjusting the number of microfibers, two different rat models with severe and mild renal failure conditions were created. The proposed delivery of radiopaque hydrogel microfibers to create an embolization model is expected to be an effective approach to control the degree of symptoms and elucidate the pathology.