Functional genomic analysis of RNA interference in C-elegans
Functional genomic analysis of RNA interference in C-elegans
复制标题
DOI：
10.1126/science.1109267
复制
发表时间：
2005-05-20
期刊：
影响因子：
56.9
通讯作者：
Ruvkun, G
中科院分区：
文献类型：
--
作者：
Kim, JK;Gabel, HW;Ruvkun, G
靶基因的RNA干扰(RNAi)是由保守的核酸酶和辅助因子处理的双链RNA(DsRNAs)触发的。为了确定RNAi所需的遗传成分，我们使用秀丽隐杆线虫的工程RNAi传感器菌株进行了全基因组筛选。RNAi筛选确定了90个基因。这些包括Piwi/Paz蛋白、Deah解旋酶、RNA结合/处理因子、染色质相关因子、DNA重组蛋白、核进出口因子以及RNAi机制的11个已知组件。我们证明这些基因中的一些也是生殖系和体细胞转基因沉默所必需的。此外，这些潜在的RNAi因子之间的物理相互作用表明与其他依赖RNA的基因调控途径有关。
RNA interference (RNAi) of target genes is triggered by doubte-stranded RNAs (dsRNAs) processed by conserved nucleases and accessory factors. To identify the genetic components required for RNAi, we performed a genome-wide screen using an engineered RNAi sensor strain of Caenorhabditis elegans. The RNAi screen identified 90 genes. These included Piwi/PAZ proteins, DEAH helicases, RNA binding/processing factors, chromatin-associated factors, DNA recombination proteins, nuclear import/export factors, and 11 known components of the RNAi machinery. We demonstrate that some of these genes are also required for germline and somatic transgene silencing. Moreover, the physical interactions among these potential RNAi factors suggest links to other RNA-dependent gene regulatory pathways.