Growth of protein 2-d crystals on supported planar lipid bilayers imaged in situ by AFM
Growth of protein 2-d crystals on supported planar lipid bilayers imaged in situ by AFM
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DOI：
10.1006/jsbi.1998.4003
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发表时间：
1998-01-01
影响因子：
3
通讯作者：
Brisson, A
中科院分区：
文献类型：
--
作者：
Reviakine, I;Bergsma-Schutter, W;Brisson, A
结晶理论，无论是三维还是二维，仍然非常有限，主要是因为缺乏提供有关基本现象的相关数据的实验方法。我们提出了一种新的实验方法，用于实时和原位跟踪固体载体上蛋白质的二维结晶过程。使用膜联蛋白V作为蛋白质与脂质基质亲和力结合的模型，我们证明了蛋白质的二维晶体可以在支撑的平面脂质双层(SPBS)上形成。原子力显微镜(AFM)使二维晶体生长过程可视化。晶体的亚分子结构以类似于2纳米的分辨率进行了表征，迄今为止在蛋白质晶体中没有观察到的缺陷被解决了。这些结果在基础科学和应用科学中具有潜在的应用价值。(C)1998年学术出版社。
Theories of crystallization, both in 3-D and 2-D, are still very limited, mainly due to the scarcity of experimental approaches providing pertinent data on elementary phenomena. We present here a novel experimental approach for following, in real time and in situ, the process of 2-D crystallization of proteins on solid supports. Using annexin V as a model of a protein binding by affinity to a lipid matrix, we show that 2-D crystals of proteins can be formed on supported planar lipid bilayers (SPBs). Atomic Force Microscopy (AFM) enables the process of 2-D crystal growth to be visualized. The submolecular organization of the crystals was characterized at a resolution of similar to 2 nanometers, and defects, hitherto not observed in protein crystals, were resolved. These results have potential applications in basic and applied sciences. (C) 1998 Academic Press.