Porcine astrovirus viremia and high genetic variability in pigs on large holdings in Croatia
Porcine astrovirus viremia and high genetic variability in pigs on large holdings in Croatia
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DOI：
10.1016/j.meegid.2012.12.027
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发表时间：
2013-03-01
影响因子：
3.2
通讯作者：
Jemersic, Lorena
中科院分区：
文献类型：
--
作者：
Brnic, Dragan;Prpic, Jelena;Jemersic, Lorena
星状病毒是新兴的病毒因子，主要在哺乳动物中具有肠道致病性，但最近已被确认在人类和水貂中具有肠道外影响。猪星状病毒被认为在猪中广泛分布和高度流行，然而其临床意义仍然值得怀疑，因为它可以在腹泻和健康猪中检测到。最近的报道暗示了猪星状病毒株之间巨大的遗传变异性，到目前为止具有五个不同的谱系。在此，我们报告猪星状病毒在克罗地亚两个大型工业控股公司饲养的不同年龄段的健康猪血液中的循环，病毒RNA血清阳性率为3.89%。这是首次在猪肠道外发现星状病毒，表明发病机制比以前认为的更复杂。部分聚合酶序列的血清来源的菌株暂时聚类到猪星状病毒谱系2和4，共享与先前描述的猪星状病毒株的高度遗传同一性。在复合粪便样本中检测到猪星状病毒株支持了结果，无论猪的类别或试验的饲养。衍生菌株的系统发育分析表明，存在先前在猪血清中检测到的猪星状病毒谱系，以及在欧洲首次报道的额外的高度遗传分歧谱系5。此外，不应排除可能的亚系的存在。本研究的结果有助于了解猪星状病毒的发病机制，尽管其可能的临床意义尚不清楚。高粪便流行率伴随着巨大的遗传多样性在一个相对狭窄的地区，强调了猪作为猪星状病毒水库的重要性，最终重组事件作为一个可能的结果。(C)2013爱思唯尔有限公司版权所有。
Astroviruses are emerging viral agents, primarily enteropathogenic in mammals, but recently have been acknowledged to have extra-intestinal implications in humans and mink. Porcine astrovirus is thought to be widely distributed and highly prevalent among pigs, nevertheless its clinical significance remains doubtful as it can be detected in diarrheic as well as in healthy pigs. Recent reports imply the immense genetic variability among porcine astrovirus strains with five distinct lineages being characterized so far. Herein, we report porcine astrovirus circulation in the blood of healthy pigs in different age categories bred on two large industrial holdings in Croatia, with viral RNA seroprevalence of 3.89%. These are the first extra-intestinal findings of astrovirus in pigs, indicating a more complex pathogenesis than previously thought. Partial polymerase sequences of serum-derived strains provisionally clustered into porcine astrovirus lineages 2 and 4, sharing high genetic identity with previously described porcine astrovirus strains. The results were supported by detecting porcine astrovirus strains in composite fecal samples, regardless of pig category or holding tested. Phylogenetic analysis of derived strains suggested the presence of porcine astrovirus lineages previously detected in pig sera with an additional highly genetically divergent lineage 5, reported for the first time in Europe. Moreover, the existence of possible sub lineages should not be excluded. The results obtained in the present study, contribute to knowledge of porcine astrovirus pathogenesis; even though it's possible clinical significance remains unclear. High fecal prevalence accompanied with vast genetic diversity on a relatively confined area, underscores the importance of pigs as porcine astrovirus reservoirs with eventual recombination events as a possible outcome. (C) 2013 Elsevier B.V. All rights reserved.