NUCLEOTIDE-SEQUENCE OF THE HUMAN CORONAVIRUS 229E RNA-POLYMERASE LOCUS
NUCLEOTIDE-SEQUENCE OF THE HUMAN CORONAVIRUS 229E RNA-POLYMERASE LOCUS
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DOI：
10.1006/viro.1993.1419
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发表时间：
1993-08-01
期刊：
影响因子：
3.7
通讯作者：
SIDDELL, SG
中科院分区：
文献类型：
--
作者：
HEROLD, J;RAABE, T;SIDDELL, SG
人类冠状病毒229E（HCV 229E）RNA聚合酶基因和基因组5'区的核苷酸序列已确定。聚合酶基因由两个大的开放阅读框 ORF1a 和 ORF1b 组成，分别包含 4086 和 2687 个密码子。 ORF1b 在 (-1) 阅读框中与 ORF1a 重叠 43 个碱基。包括包含 ORF1a/ORF1b 连接的 HCV 229E 序列的 SP6 转录物的体外翻译表明，ORF1b 的表达可以通过核糖体移码介导。将ORF1a（454,200分子量）和ORF1a/1b（754,200分子量）的预测翻译产物与传染性支气管炎病毒（IBV）和鼠肝炎病毒（MHV）的预测RNA聚合酶基因产物进行比较，并鉴定了保守的结构特征和推定的功能域。该分析完成了 HCV 229E 基因组的核苷酸序列。
The nucleotide sequence of the human coronavirus 229E (HCV 229E) RNA polymerase gene and the 5′ region of the genome has been determined. The polymerase gene is comprised of two large open reading frames, ORF1a and ORF1b, that contain 4086 and 2687 codons, respectively. ORF1b overlaps ORF1a by 43 bases in the (-1) reading frame. Thein vitrotranslation of SP6 transcripts which include HCV 229E sequences encompassing the ORF1a/ORF1b junction show that expression of ORF1b can be mediated by ribosomal frame-shifting. The predicted translation products of ORF1a (454,200 molecular weight) and ORF1a/1b (754,200 molecular weight) have been compared to the predicted RNA polymerase gene products of infectious bronchitis virus (IBV) and murine hepatitis virus (MHV) and conserved structural features and putative functional domains have been identified. This analysis completes the nucleotide sequence of the HCV 229E genome.