Ciprofloxacin at low levels disrupts colonization resistance of human fecal microflora growing in chemostats
Ciprofloxacin at low levels disrupts colonization resistance of human fecal microflora growing in chemostats
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DOI：
10.1016/j.yrtph.2004.08.005
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发表时间：
2004-12-01
影响因子：
3.4
通讯作者：
Bartholomew, MJ
中科院分区：
文献类型：
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作者：
Carman, RJ;Simon, MA;Bartholomew, MJ
研究了不同浓度环丙沙星（CI）对人肠道菌群对克道沟沙门氏菌NCTC 12173定殖耐药性的体外影响。从单个人类粪便池中接种疫苗，在趋化器中建立了四个稳定的微生物群落。三种趋化剂暴露于CI（分别为0.1、0.43和5马克杯/毫升）；一个是无毒品控制组。每个社区的CR是通过连续三次每天喂108只克度菇来测试的，每次喂给1 mL。无药化抑素没有定植。同样，暴露于0.1杯/毫升CI后，CR没有损失，S. kedougou也没有定植。相反，暴露于0.43杯/毫升和5杯/毫升的菌群都遭受了CR的损失，这些趋化因子被定殖。与5马克杯/毫升相比，0.43马克杯/毫升条件下的克杜鹃生长速度更快，数量也更高。一种可能的解释是CI对攻毒菌株和CR都有剂量依赖效应。因此，在较高水平下，即使CR被CI破坏，攻毒菌株的生长也会受到影响。由于暴露于CI引起了大肠杆菌数量的剂量依赖性减少[Reg。杂志。我们的新数据表明，大肠杆菌可能有助于对抗沙门氏菌的CR。我们进一步得出结论，即使在低于治疗期间达到的粪便水平下，CI也可能对人体肠道菌群产生足够的影响，从而促进S. kedougou定植。(C) 2004爱思唯尔公司版权所有。
We studied the in vitro effects of a range of ciprofloxacin (CI) concentrations on the human intestinal flora's colonization resistance (CR) to Salmonella kedougou NCTC 12173. Four steady state microbial communities were established in chemostats using inocula from a single pool of human feces. Three chemostats were exposed to CI (0.1, 0.43 and 5 mug/mL, respectively); one served as a no-drug control. The CR of each community was tested by three successive daily challenges of 108 S. kedougou, each delivered in a 1 mL bolus. There was no colonization of the no-drug chemostat. Likewise, after exposure to only 0.1 mug/mL CI there was no loss of CR and S. kedougou did not colonize. Conversely, both the 0.43 and the 5 mug/mL-exposed floras suffered a loss of CR and these chemostats were colonized. S. kedougou overgrew faster and reached higher counts in the presence of 0.43 than it did in the presence of 5 mug/mL. One possible explanation is that CI had a dose-dependent effect on both the challenge strain and CR. Thus, at higher levels, even though CR was disrupted by CI, so too was the growth of the challenge strain. Since exposure to CI elicited a dose-dependent reduction in Escherichia coli counts [Reg. Pharmacol. Toxicol. 33 (2001) 276] our new data suggest that E. coli may contribute to the CR against salmonella. We further conclude that, even at fecal levels below those reached during therapy, CI may impact the human gut flora sufficiently to facilitate colonization by S. kedougou. (C) 2004 Elsevier Inc. All rights reserved.