Cytokinins Stimulate Plasmodesmatal Transport in Leaves.
Cytokinins Stimulate Plasmodesmatal Transport in Leaves.
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DOI：
10.3389/fpls.2021.674128
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发表时间：
2021
影响因子：
5.6
通讯作者：
Brunkard JO
中科院分区：
文献类型：
--
作者：
Horner W;Brunkard JO
植物细胞通过胞间连丝（PD）相连，胞间连丝是细胞壁中的纳米级通道，能让多种胞质分子在相邻细胞间移动。尽管影响PD运输的信号通路范围尚未全面界定，但PD运输与植物生理及发育紧密协调。已知包括水杨酸（SA）和生长素在内的几种植物激素可调节PD运输，但其他激素的作用尚未明确。在本研究中，我们提供的证据表明细胞分裂素可促进叶片中的PD运输。通过在本氏烟草表皮进行绿色荧光蛋白（GFP）移动分析，我们发现，当向叶片提供生理相关浓度的外源细胞分裂素，或细胞分裂素响应的正调节因子拟南芥组氨酸磷酸转移蛋白5（AHP5）过表达时，PD运输显著增加。随后我们证明，使细胞分裂素受体拟南芥组氨酸激酶3（AHK3）或AHK4沉默，或过表达细胞分裂素信号的负调节因子AAHP6，会显著降低PD运输。对PD运输增加的突变体（ise1 - 4）进行的转录组分析支持了这些结果，该分析显示出细胞分裂素信号增强的迹象。我们得出结论，细胞分裂素有助于植物中PD运输的动态变化，这将在植物生物学的多个方面产生影响，包括分生组织模式形成与发育、库源转换的调控以及植物激素间的相互作用。
Plant cells are connected by plasmodesmata (PD), nanoscopic channels in cell walls that allow diverse cytosolic molecules to move between neighboring cells. PD transport is tightly coordinated with physiology and development, although the range of signaling pathways that influence PD transport has not been comprehensively defined. Several plant hormones, including salicylic acid (SA) and auxin, are known to regulate PD transport, but the effects of other hormones have not been established. In this study, we provide evidence that cytokinins promote PD transport in leaves. Using a green fluorescent protein (GFP) movement assay in the epidermis of Nicotiana benthamiana, we have shown that PD transport significantly increases when leaves are supplied with exogenous cytokinins at physiologically relevant concentrations or when a positive regulator of cytokinin responses, ARABIDOPSIS HISTIDINE PHOSPHOTRANSFER PROTEIN 5 (AHP5), is overexpressed. We then demonstrated that silencing cytokinin receptors, ARABIDOPSIS HISTIDINE KINASE 3 (AHK3) or AHK4 or overexpressing a negative regulator of cytokinin signaling, AAHP6, significantly decreases PD transport. These results are supported by transcriptomic analysis of mutants with increased PD transport (ise1–4), which show signs of enhanced cytokinin signaling. We concluded that cytokinins contribute to dynamic changes in PD transport in plants, which will have implications in several aspects of plant biology, including meristem patterning and development, regulation of the sink-to-source transition, and phytohormone crosstalk.
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影响因子：
9.5
作者：
Brunkard JO;Runkel AM;Zambryski PC
通讯作者：
Zambryski PC
DOI：
10.1073/pnas.0808717106
发表时间：
2009-03-03
影响因子：
11.1
作者：
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影响因子：
11.6
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影响因子：
64.8
作者：
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影响因子：
9.2
作者：
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通讯作者：
Helariutta, Yka