Continuous theta burst stimulation facilitates the clearance efficiency of the glymphatic pathway in a mouse model of sleep deprivation
Continuous theta burst stimulation facilitates the clearance efficiency of the glymphatic pathway in a mouse model of sleep deprivation
复制标题
连续θ爆发刺激促进睡眠剥夺小鼠模型中类淋巴通路的清除效率
DOI：
10.1016/j.neulet.2017.05.064
复制
发表时间：
2017-07-13
影响因子：
2.5
通讯作者：
Xu, Guang-qing
中科院分区：
文献类型：
--
作者：
Liu, Dong-xu;He, Xia;Xu, Guang-qing
睡眠剥夺（SD）是一种与多种神经系统疾病相关的常见疾病，对情绪和认知功能有负面影响。持续θ波爆发刺激（cTBS）已知可以改善正常情况下的认知和情绪功能，以及各种类型的功能障碍，但其机制尚不清楚。我们采用双光子体内成像技术探讨cTBS对正常和SD C57BL/6J小鼠淋巴通路清除率的影响。免疫荧光法检测水通道蛋白-4 （AQP4）极化。焦虑样行为采用野外测试进行测量。我们发现SD降低了沿血管周围空间（PVS）的内流效率，干扰了AQP4极化并诱导了焦虑样行为。CTBS显著减轻了SD动物通常引起的溶质清除效率下降，恢复了AQP4极化的缺失，改善了SD动物的焦虑样行为。我们的研究结果表明cTBS有可能防止睡眠障碍引起的神经元功能障碍。(C) 2017 Elsevier B.V.版权所有
Sleep deprivation (SD) is a common condition associated with a variety of nervous system diseases, and has a negative impact on emotional and cognitive function. Continuous theta burst stimulation (cTBS) is known to improve cognition and emotion function in normal situations as well as in various types of dysfunction, but the mechanism remains unknown. We used two-photon in vivo imaging to explore the effect of cTBS on glymphatic pathway clearance in normal and SD C57BL/6J mice. Aquaporin-4 (AQP4) polarization was detected by immunofluorescence. Anxiety-like behaviors was measured using open field tests. We found that SD reduced influx efficiency along the peri-vascular space (PVS), disturbed AQP4 polarization and induced anxiety-like behaviors. CTBS significantly attenuated the decrease in efficiency of solute clearance usually incurred with SD, restored the loss of AQP4 polarization and improved anxiety like behavior in SD animals. Our results implied that cTBS had the potential to protect against neuronal dysfunction induced by sleep disorders. (C) 2017 Elsevier B.V. All rights reserved.