Assessment of oocyte quality following repeated gonadotropin stimulation in the mouse
Assessment of oocyte quality following repeated gonadotropin stimulation in the mouse
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DOI：
10.1095/biolreprod.102.008656
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发表时间：
2003-03-01
影响因子：
3.6
通讯作者：
Albertini, DF
中科院分区：
文献类型：
--
作者：
Combelles, CMH;Albertini, DF
本研究评估了重复卵巢刺激对卵母细胞质量的影响。雌性小鼠每隔 1 周用 eCG 和 hCG 刺激一次，持续 4 周。每周刺激后，对生殖囊泡 (GV) 阶段卵母细胞的大小、体细胞关联和染色质组织进行评估。此外，在 GV 和体内 (IVO) 或体外 (IVM) 成熟卵母细胞中监测 ATP 含量和减数分裂能力的表达。在体外受精和胚胎培养后测定排卵卵母细胞的发育能力，并在重复刺激周期交配后评估生殖结果。在 GV 卵母细胞中，与重复刺激和单周期刺激相比，体细胞关联程度、大小和转录抑制时间发生了变化。 IVO 卵母细胞的减数分裂能力表达不受影响，而 IVM 卵母细胞的减数分裂能力随着重复刺激而逐渐降低。未成熟卵母细胞和IVO卵母细胞的ATP含量随着重复刺激而降低。尽管在一个刺激周期后，IVM 卵母细胞中的 ATP 含量低于 IVO 卵母细胞，但 IVM 卵母细胞在整个刺激周期中表现出稳定的 ATP 水平。最后，重复刺激后回收的 IVO 卵母细胞的体外发育能力没有显着差异，并且在体内，在经历重复刺激的动物交配后观察到相似的植入和吸收率。因此，尽管重复刺激对卵泡卵母细胞质量的特定参数产生可测量的后果，但体内可能存在补偿机制以优化小鼠排卵卵母细胞的发育能力。
The present study assessed the effects of repeated ovarian stimulation on oocyte quality. Female mice were stimulated with eCG and hCG at 1-wk intervals for 4 wk. Germinal vesicle (GV)stage oocytes were evaluated in relation to size, somatic cell association, and chromatin organization after each week of stimulation. In addition, ATP content and expression of meiotic competence were monitored in GV and in vivo (IVO) or in vitro (IVM)-matured oocytes. The developmental competence of ovulated oocytes was determined after in vitro fertilization and embryo culture, and reproductive outcome was evaluated after mating following repeated cycles of stimulation. In GV oocytes, the degree of somatic cell association, size, and timing of transcriptional repression were altered when comparing repeated with single cycle(s) of stimulation. Meiotic competence expression was unaffected for IVO oocytes while IVM oocytes exhibited a progressive decrease in meiotic competence with repeated stimulation. The ATP content of immature and IVO oocytes decreased with repeated stimulation. Although after one cycle of stimulation ATP content was lower in IVM than IVO oocytes, IVM oocytes exhibited stable levels of ATP across cycles of stimulation. Last, the in vitro developmental competence of IVO oocytes retrieved after repeated stimulation was not significantly different, and in vivo, similar implantation and resorption rates were observed following mating of animals subjected to repeated stimulation. Therefore, despite measurable consequences of repeated stimulation on specific parameters of follicular oocyte quality, compensatory mechanisms may exist in vivo to optimize the developmental competence of ovulated oocytes in the mouse.