A comprehensive study of glucose transfer in the human small intestine using an in vitro intestinal digestion system (i-IDS) based on a dialysis membrane process
A comprehensive study of glucose transfer in the human small intestine using an in vitro intestinal digestion system (i-IDS) based on a dialysis membrane process
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DOI：
10.1016/j.memsci.2018.07.080
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发表时间：
2018-10-15
影响因子：
9.5
通讯作者：
Troncoso, Elizabeth
中科院分区：
文献类型：
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作者：
Gim-Krumm, Minghai;Donoso, Pablo;Troncoso, Elizabeth
人体消化是一个复杂的过程，涉及几种现象：化学和酶反应，吸收，流体动力学过程和传质。确定不同营养素生物利用度的最准确方法是通过人体研究。然而，有道德，经济和技术的原因，限制其应用。为了提供一个新的实验原型和现象学模型来研究营养物质在人体小肠中的吸收，构建了一个基于中空纤维透析膜过程的体外小肠消化系统（i-IDS），并在不同的进料流量/透析液流量比的操作条件下进行了测试。本文提出的透析膜过程允许模拟和外推人体小肠中葡萄糖转移过程的结果，其中葡萄糖传质在25和40 min之间的时间范围内达到约90%。数学模型与实验结果进行了验证，获得了良好的一致性（均方根误差，% RMS，在3%和19%之间）。实验条件下的总体渗透率范围在3.85 x 10（-6）和4.86 x 10（-6）m（3）/s之间。使用现象学模型将葡萄糖吸收的实验结果外推到在人小肠中发现的那些，其中在人体研究中发现葡萄糖吸收的结果之间具有良好的一致性。这是第一个实验和现象学的方法，可以获得更好的知识，在人体小肠中发现的营养吸收的复杂的传质过程。
Human digestion is a complex process that involves several phenomena: chemical and enzymatic reactions, absorption, hydrodynamic processes, and mass transfer. The most accurate way to determine the bioavailability of different nutrients is through human studies. However, there are ethical, economic, and technical reasons that restrict their application. Aiming at contributing with a new experimental prototype and phenomenological model to study nutrient absorption in the human small intestine, an in vitro intestinal digestion system (i-IDS), based on a hollow fiber dialysis membrane process, was constructed and tested under different operational conditions of feed flow/dialysate flow ratio. This dialysis membrane process here presented, allowed to simulate and extrapolate results of the process of glucose transfer in the human small intestine, where the glucose mass transfer reached around 90% at times ranging between 25 and 40 min. The mathematical model was validated with respect to the experimental results, obtaining a good agreement (root mean square error, % RMS, between 3% and 19%) among them. Overall permeabilities for the experimental conditions ranged between 3.85 x 10(-6) and 4.86 x 10(-6) m(3)/s. The experimental results of glucose absorption were extrapolated to those found in the human small intestine using a phenomenological model, where a good agreement among results of glucose absorption was found in human studies. This first experimental and phenomenological approach allows to acquire a better knowledge of the complex mass transfer processes found in human small intestine for nutrient absorption.