Population dynamics of Theileria sergenti in persistently infected cattle and vector ticks analysed by a polymerase chain reaction
Population dynamics of Theileria sergenti in persistently infected cattle and vector ticks analysed by a polymerase chain reaction
复制标题
DOI：
10.1017/s0031182000076897
复制
发表时间：
1996-05-01
期刊：
影响因子：
2.4
通讯作者：
Onuma, M
中科院分区：
文献类型：
--
作者：
Kubota, S;Sugimoto, C;Onuma, M
瑟氏泰勒虫Shintoku股票由2个寄生虫种群携带2个等位基因形式的p33/32，免疫显性的梨形表面蛋白。利用等位基因特异性聚合酶链反应（PCR）分析了牛和蜱媒之间寄生虫传代期间以及个体小牛持续感染期间寄生虫种群的变化。通过接种子孢子稳定体或寄生红细胞，从感染了Shintoku原种的小牛的蜱虫中制备寄生虫DNA，并从感染了Shintoku原种的蜱虫中制备子孢子稳定体。在传播过程中，从小牛到媒介蜱和从感染蜱到小牛的优势寄生虫种群的变化。在牛持续感染数月期间，寄生虫种群变化也很明显，这种变化被认为是在宿主免疫压力下发生的。本研究的结果表明，不同形式的p33/32的表达可能在哺乳动物宿主内的寄生虫持久性及其从蜱媒介的传播中发挥作用。
Theileria sergenti Shintoku stock consists of 2 parasite populations bearing 2 allelic forms of p33/32, an immunodominant piroplasm surface protein. Parasite population changes during parasite passages among cattle and tick vectors, and during persistent infection in individual calves were analysed by using allele-specific polymerase chain reaction (PCR). The parasite DNAs were prepared from piroplasms from calves which had been infected with Shintoku stock by inoculation of sporozoite stabilates or parasitized erythrocytes, and from sporozoite stabilates which had been prepared from Shintoku stock-infected ticks. Changes in a dominant parasite population were demonstrated during transmission from calves to vector ticks and from infected ticks to calves. Parasite population changes were also apparent during persistent infection in cattle over several months, and this change is thought to occur under host immune pressure. The results of this study indicate that expression of diverse forms of p33/32 may play a role in parasite persistence within mammalian hosts and its transmission from tick vector.