Development of biopolymer nanocomposite for silver nanoparticles and Ciprofloxacin controlled release
Development of biopolymer nanocomposite for silver nanoparticles and Ciprofloxacin controlled release
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DOI：
10.1016/j.ijbiomac.2014.09.020
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发表时间：
2015-01-01
影响因子：
8.2
通讯作者：
Castro, Guillermo R.
中科院分区：
文献类型：
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作者：
Islan, German A.;Mukherjee, Arup;Castro, Guillermo R.
筛选含有稳定在瓜尔胶烷基胺（GGAA）和环丙沙星（Cip）中的银纳米颗粒（Ag-NPs）的生物聚合物凝胶珠，以获得具有两种抗菌剂的控制释放特性的新型纳米复合物。选择的由海藻酸盐/高甲氧基果胶（HMP）/GGAA（4：4：1）组成的基质能够以高于70%的包封率共掺入Ag-NPs和Cip。SEM图像显示生物聚合物与货物之间具有良好的粘结性和相容性。珠粒提供了在酸性pH下针对Ag-NP降解的保护，并且HMP将发挥提供疏水区域的关键作用。虽然Cip释放曲线显示pH独立的扩散过程，但Ag-NPs的释放仅限于基质的可蚀性。钙平衡剂和/或海藻酸降解酶可以调节释放曲线。在液体培养基中测试珠的杀菌活性，显示抗微生物剂对铜绿假单胞菌、大肠杆菌、蜡样芽孢杆菌和金黄色葡萄球菌之间的协同作用。TEM图像证实了Ag-NPs与细菌表面的相互作用，随后是膜损伤。结果表明，纳米复合材料基质作为口服治疗由多重耐药和未知微生物引起的肠道感染性疾病的有前途的系统，因为Cip和Ag-NP都能够以活性形式到达肠道。(C)2014爱思唯尔有限公司版权所有。
Screening of biopolymeric gel beads containing Silver NanoParticles (Ag-NPs) stabilized in Guar Gum Alkyl Amine (GGAA) and Ciprofloxacin (Cip) was carried out in order to obtain a novel nanocomposite with controlled release profile of both antimicrobians. The selected matrix composed of Alginate/High Methoxyl Pectin (HMP)/GGAA (4:4:1) was able to co-incorporate Ag-NPs and Cip with encapsulation efficiency higher than 70%. SEM images revealed good cohesivity and compatibility between the biopolymers and the cargos. Beads provided protection against Ag-NPs degradation at acidic pHs and HMP would played a key role providing hydrophobic regions. While Cip release profile showed a pH independent diffusional process, Ag-NPs release was restricted to matrix erodability. Calcium quelating agents and/or alginate degrading enzymes could modulate the release profile. The bactericidal activity of beads was tested in liquid medium, showing cooperative effects between the antimicrobials against Pseudomonas aeruginosa, Escherichia coli, Bacillus cereus and Staphylococcus aureus. TEM images confirmed interaction of Ag-NPs with bacterial surfaces followed by membrane damage. Results suggested the nanocomposite matrix as a promising system for oral treatment of intestinal infectious diseases caused by multidrug resistant and unknown microorganisms, since both Cip and Ag-NPs would be able to reach intestine in the active form. (C) 2014 Elsevier B.V. All rights reserved.