Protein Kinase A Modulates Cdc25B Activity During Meiotic Resumption of Mouse Oocytes
Protein Kinase A Modulates Cdc25B Activity During Meiotic Resumption of Mouse Oocytes
复制标题
蛋白激酶 a 在小鼠卵母细胞减数分裂恢复过程中调节 Cdc25B 活性
DOI：
10.1002/dvdy.21799
复制
发表时间：
2008-12-01
影响因子：
2.5
通讯作者：
Yu, Bing-Zhi
中科院分区：
文献类型：
--
作者：
Zhang, Yang;Zhang, Zhe;Yu, Bing-Zhi
蛋白激酶A（PKA）在维持减数分裂停滞中起着关键作用。然而，PKA下游的步骤在很大程度上仍然未知。本研究探讨PKA/Cdc 25 B信号通路对小鼠卵母细胞减数分裂恢复的调控作用。注射编码Cdc 25 b-S321 A的mRNA具有比Cdc 25 b-WT更强的成熟诱导能力。当与PKA抑制剂共注射时，Cdc 25 B-WT具有与Cdc 25 B-S321 A相似的活性。同时，用磷酸化丝氨酸321特异性抗体通过蛋白质印迹法检测到Cdc 25 B-S321在生殖囊泡（GV）卵母细胞中的磷酸化，并且当卵母细胞重新进入减数分裂细胞周期时，条带消失。此外，Cdc 25 B-WT在GV破裂（GVBD）前不久易位到细胞核，而磷酸化Cdc 25 B-S321仅在细胞质中表达，并且在GVBD卵母细胞中无法检测到信号。综上所述，这些数据表明Cdc 25 B-丝氨酸321是潜在的PKA靶标，并且Cdc 25 B亚细胞定位决定了其在小鼠卵母细胞中维持GV停滞过程中的功能。发展动力学237：3777-3786，2008年。© 2008 Wiley利斯公司
Protein kinase A (PKA) play a critical role in maintaining the meiotic arrest. However, the steps downstream of PKA remain largely unknown. In this study, we investigated the regulation of meiotic resumption by PKA/Cdc25B pathway in mouse oocytes. Injection of mRNA coding for Cdc25b‐S321A had a more potent maturation‐inducing ability than Cdc25b‐WT. When co‐injected with PKA inhibitor, Cdc25B‐WT had similar activities with Cdc25B‐S321A. Meanwhile, the phosphorylation of Cdc25B‐S321 was detected in germinal vesicle (GV) oocytes by Western blotting with a phospho‐Ser321‐specific antibody and the band disappeared when oocytes reenter into the meiotic cell cycle. Furthermore, Cdc25B‐WT translocated to the nucleus shortly before GV breakdown (GVBD), whereas phosphorylated Cdc25B‐S321 expressed exclusively in the cytoplasm and the signal could not be detected in GVBD oocytes. Taken together, these data indicate that Cdc25B‐Serine321 is the potential PKA target and Cdc25B subcellular localization determines its function during the process of maintaining GV arrest in mouse oocytes. Developmental Dynamics 237:3777–3786, 2008. © 2008 Wiley‐Liss, Inc.