PROTEINS RELEASED FROM MYCOBACTERIUM-TUBERCULOSIS DURING GROWTH
PROTEINS RELEASED FROM MYCOBACTERIUM-TUBERCULOSIS DURING GROWTH
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DOI：
10.1128/iai.59.6.1905-1910.1991
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发表时间：
1991-06-01
影响因子：
3.1
通讯作者：
HERON, I
中科院分区：
文献类型：
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作者：
ANDERSEN, P;ASKGAARD, D;HERON, I
结核分枝杆菌在生长过程中分泌的蛋白质被认为是预防结核病的保护性免疫的重要因素。我们研究了结核分枝杆菌在浓缩液中的生长情况。从细菌中释放异柠檬酸脱氢酶作为自溶的标志，并在对数生长后期观察到。采用酶联免疫吸附试验和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法研究培养过程中蛋白质的释放情况。定义了三个主要的蛋白质组，它们在释放和在完整细菌中的位置方面有明显的差异。定义了一种不含自溶产物的短期滤液，发现其由33种主要成分组成。用单抗鉴定了5种蛋白。滤液中检测到明显的超氧化物歧化酶活性。该酶被纯化，并被鉴定为短期滤液的主要成分。
Proteins secreted from Mycobacterium tuberculosis during growth are believed to be important for protective immunity against tuberculosis. We have investigated the growth of M. tuberculosis in an enriched liquid medium. The release of isocitrate dehydrogenase from the bacilli served as a marker of autolysis and was observed during the late logarithmic growth phase. The release of proteins during the culture period was investigated by enzyme-linked immunosorbent assay and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Three major groups of proteins, which differed markedly with respect to profile of release and location in intact bacilli, were defined. A short-term filtrate devoid of autolytic products was defined and found to be composed of 33 major components. Five proteins were identified by monoclonal antibodies. Pronounced superoxide dismutase activity was detected in the filtrate. The enzyme was purified and identified as a dominating component of short-term filtrate.