Complete sequencing of a gibbon hepatitis B virus genome reveals a unique genotype distantly related to the chimpanzee hepatitis B virus
Complete sequencing of a gibbon hepatitis B virus genome reveals a unique genotype distantly related to the chimpanzee hepatitis B virus
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DOI：
10.1006/viro.1996.0181
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发表时间：
1996-04-01
期刊：
影响因子：
3.7
通讯作者：
Magnius, LO
中科院分区：
文献类型：
--
作者：
Norder, H;Ebert, JW;Magnius, LO
我们已经对一种B型肝炎病毒（HBV）株的全基因组进行了测序，该病毒株是从患有慢性B型肝炎的长颈鹿传播给随后发展为急性B型肝炎的黑猩猩的。基因组长3，182个核苷酸，具有与其他哺乳动物嗜肝DNA病毒相同的遗传组织，并包括其特征。因此，该病毒的调控元件、同向重复序列和四个开放阅读框（ORF）都得以保留，尽管存在影响所有ORF的氨基酸替换。在编码B型肝炎表面抗原（HBsAg）的S基因内，根据先前的血清学结果，可将亚型推断为ayw3。P基因有25个氨基酸的替换，其中12个位于间隔区，该区域也编码前S蛋白，是基因组中与其他HBV毒株相比最不同的部分。与其他嗜肝DNA病毒序列的比较显示，与先前描述的HBV基因型相比，giganase HBV的基因组是独特的。它与黑猩猩HBV毒株最相似，在全基因组水平上与其具有90.3%的核酸同源性，在对应于HBsAg的S基因区水平上具有96.3%的同源性，尽管与后者病毒相比是不同的基因型。使用5种不同的系统发育树构建算法进行的分析显示，99%以上的bootstrap支持长颈鹿和黑猩猩HBV被归类为人类HBV毒株，并且它们代表了比基因型F的HBV毒株更晚的分支。然而，在大多数树状图中，长颈鹿和黑猩猩株都代表了早期谱系，表明这些病毒是各自宿主的固有病毒，而不是最近从人类获得的病毒。
We have sequenced the complete genome of a hepatitis B virus (HBV) strain that was transmitted from a gibbon with chronic hepatitis B to a chimpanzee that subsequently developed acute hepatitis B. The genome was 3,182 nucleotides long and had a genetic organization identical to and including the characteristics of other mammalian hepadnaviruses. Thus, the regulatory elements, the direct repeats, and the four open reading frames (ORFs) of this virus were all maintained, although there were amino acid substitutions affecting all the ORFs. Within the S gene encoding for the hepatitis B surface antigen (HBsAg), the subtype could be deduced as ayw3 in accordance with previous serological results. There were 25 amino acid substitutions affecting the P gene, 12 of which were within the spacer region, This region, which was the most divergent part of the genome compared to other HBV strains, also encodes for the pre-S proteins. A comparison with sequences of other hepadnaviruses revealed that the genome of gibbon HBV was unique as compared to previously described HBV genotypes. It was most similar to the chimpanzee HBV strain with which it shared 90.3% nucleic acid homology at the level of the complete genome and 96.3% homology at the level of the S-gene region corresponding to HBsAg, although being a distinct genotype as compared to the latter virus. Analyses performed using five different algorithms for phylogenetic tree construction showed more than 99% bootstrap support for the gibbon and the chimpanzee HBV to be grouped within the human HBV strains and that they represented later offshoots than the HBV strains of genotype F. However, in most of the dendrograms both the gibbon and the chimpanzee strains represented early lineages, indicating that these viruses are indigenous to their respective hosts and not recent acquisitions from man. (C) 1996 Academic Press, Inc.