Transcriptional profiling of human herpesvirus type B (HHV-6B) in an adult T cell leukemia cell line as in vitro model for persistent infection
Transcriptional profiling of human herpesvirus type B (HHV-6B) in an adult T cell leukemia cell line as in vitro model for persistent infection
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DOI：
10.1016/j.bbrc.2005.01.090
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发表时间：
2005-04-01
影响因子：
3.1
通讯作者：
Ohyashiki, K
中科院分区：
文献类型：
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作者：
Ohyashiki, JH;Takaku, T;Ohyashiki, K
人类疱疹病毒 6 (HHV-6) 存在于 90% 以上的人体中，已知会导致免疫受损患者（例如移植患者）感染性疾病。为了阐明 HHV-6 基因表达模式在各种类型的 HHV-6B 感染中的可能作用，我们试图确定病毒 DNA 微阵列是否可用于使用包含 97 个已知 HHV-6B 开放阅读框的 HHV-6B DNA 微阵列来详细表征病毒转录。一部分基因在持续性感染中优先表达：U16（IE-B，反式激活蛋白，US22 基因家族），U18（IE-B。HCMV IE 糖蛋白同源物），U20（糖蛋白），U27（DNA 聚合酶持续性反式激活蛋白），U82（gL，gH 辅助蛋白），U83（趋化因子），U85（OX-2 同源性，糖蛋白），U90分别是（IE-A）和U94（反式激活因子）。尽管每个HHV-6B的功能尚不完全清楚，但我们的研究表明，对HHV-6B转录的全面分析不仅有助于阐明病毒的发病机制，而且有助于开发抗病毒药物的新策略。 (C) 2005 Elsevier Inc. 保留所有权利。
Human herpesvirus 6 (HHV-6), which is present in more than 90% of the human, is known to cause infectious diseases in immuno-compromised patients, e.g., transplant patients. To clarify the possible role of the pattern of expression of HHV-6 genes in various types of HHV-6B infection, we sought to determine whether or not viral DNA microarray could be used for detailed characterization of viral transcription using a HHV-6B DNA microarray that contains 97 known open reading frames of HHV-6B. A subset of genes are preferentially expressed in persistent infection: U16 (IE-B, transactivator, US22 gene family), U18 (IE-B. homolog to HCMV IE glycoprotein), U20 (glycoprotein), U27 (DNA polymerase processivity transactivator), U82 (gL, gH accessory protein), U83 (chemokine), U85 (OX-2 homology, glycoprotem), U90 (IE-A), and U94 (transactivator), respectively. Although the function of each HHV-6B is not fully understood, our study suggests that comprehensive analysis of HHV-6B transcription is useful not only to clarify the pathogenesis of the virus but also to develop new strategies for anti-viral drugs. (C) 2005 Elsevier Inc. All rights reserved.