Radiation-Induced Bystander Signaling from Somatic Cells to Germ Cells in Caenorhabditis elegans
Radiation-Induced Bystander Signaling from Somatic Cells to Germ Cells in Caenorhabditis elegans
复制标题
线虫中辐射诱导的从体细胞到生殖细胞的旁观者信号传导
DOI：
10.1667/rr3218.1
复制
发表时间：
2013-09-01
影响因子：
3.4
通讯作者：
Wu, Lijun
中科院分区：
文献类型：
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作者：
Guo, Xiaoying;Sun, Jie;Wu, Lijun
近年来，辐射诱导的旁观者效应（里贝）在小鼠体内模型中得到了研究，清楚地证明了体细胞间的旁观者效应。然而，目前还没有证据表明体内动物模型中体细胞和生殖细胞之间存在里贝。本研究以秀丽隐杆线虫为模型动物，探讨了体细胞向生殖细胞的旁观者信号传递及其机制。C.秀丽线虫的体型允许精确的微束照射，并且相对于目前采用的小鼠模型，用于遗传解剖的丰富的突变株使其对于这种分析是理想的。结果表明，照射后咽球和尾囊可使C。elegans增强了旁观者性腺中生殖细胞的凋亡水平。后咽球的照射也增加了旁观者生殖细胞的DNA损伤水平和受照射的蠕虫的F1子代的基因组不稳定性，表明即使只有亲本的体细胞暴露于电离辐射（IR），后代也存在潜在的致癌风险。DNA损伤诱导的生殖细胞死亡机制和MAPK信号通路均参与了微束诱导的旁观者信号通路诱导生殖细胞凋亡的过程，表明从体细胞到生殖细胞的旁观者效应是多条信号通路之间的复杂协作。(C)2013年，辐射研究学会
Recently, radiation-induced bystander effects (RIBE) have been studied in mouse models in vivo, which clearly demonstrated bystander effects among somatic cells. However, there is currently no evidence for RIBE between somatic cells and germ cells in animal models in vivo. In the current study, the model animal Caenorhabditis elegans was used to investigate the bystander signaling from somatic cells to germ cells, as well as underlying mechanisms. C. elegans body size allows for precise microbeam irradiation and the abundant mutant strains for genetic dissection relative to currently adopted mouse models make it ideal for such analysis. Our results showed that irradiation of posterior pharynx bulbs and tails of C. elegans enhanced the level of germ cell apoptosis in bystander gonads. The irradiation of posterior pharynx bulbs also increased the level of DNA damage in bystander germ cells and genomic instability in the F1 progeny of irradiated worms, suggesting a potential carcinogenic risk in progeny even only somatic cells of parents are exposed to ionizing radiation (IR). It was also shown that DNA damage-induced germ cell death machinery and MAPK signaling pathways were both involved in the induction of germ cell apoptosis by microbeam induced bystander signaling, indicating a complex cooperation among multiple signaling pathways for bystander effects from somatic cells to germ cells. (C) 2013 by Radiation Research Society