Changes in serum cytokines in response to musculoskeletal surgical trauma.
Changes in serum cytokines in response to musculoskeletal surgical trauma.
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DOI：
10.1186/1756-0500-7-128
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发表时间：
2014-03-07
影响因子：
1.8
通讯作者：
Lyngstadaas, Staale Petter
中科院分区：
文献类型：
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作者：
Reikeras, Olav;Borgen, Pal;Lyngstadaas, Staale Petter
背景：创伤会诱发局部和随后的全身炎症反应，当细胞因子的产生失调时，可能会发生具有潜在致命结果的全身炎症反应综合征。了解细胞因子网络的生理机制将有助于更好地理解病理状况。方法：我们分析了 20 名全髋关节置换术患者血清中的 30 种细胞因子。通过多重抗体珠试剂盒评估术后 6 天的细胞因子释放，并与术前值进行比较。 结果：手术导致术后 6 天的血清 IL-2R 水平、术后 6 小时和术后 1 天的 IL-6 水平、术后 6 小时的 IL-8 水平、术后 6 小时和术后 1 天的 IL-16 水平显着增加。手术结束时血清 IL-1Rα 水平、手术结束时和术后 6 小时的 IL-12 水平以及术后各阶段的嗜酸细胞活化趋化因子 (Eotaxin) 水平均显着降低。结论：主要发现是促炎细胞因子 IL-6、IL-8、IL-16 的全身水平显着升高，而 IL-12 显着降低。除此之外，全身细胞因子动力学发生适度变化，并且抗炎细胞因子没有显着表达。
BACKGROUND: Trauma induces local and subsequent systemic inflammatory reactions, and when the cytokine production is deregulated, a systemic inflammatory response syndrome with a potentially lethal outcome can occur. The understanding of the physiological mechanism of the cytokine network would be useful to better comprehend pathological conditions.METHODS: We analysed a panel of 30 cytokines in the serum of 20 patients operated with total hip replacement. Cytokine release was assessed postoperatively up to 6days by a multiplex antibody bead kit and compared to pre-operative values.RESULTS: Surgery induced significant increments in serum levels of IL-2R at 6days after surgery, in levels of IL-6 at 6hours after surgery and at 1day after surgery, in levels of IL-8 at 6hours after surgery, in levels of IL-16 at 6hours and at 1day after surgery. Significant decreases in serum levels of IL-1Ralpha were found at the end of surgery, in levels of IL-12 at the end of surgery and at 6hours after, and in levels of Eotaxin during all phases of the postoperative course.CONCLUSIONS: The major findings were significant increases in systemic levels of the pro-inflammatory cytokines IL-6, IL-8, IL-16, while IL-12 was significantly decreased. Otherwise there were modest changes in the systemic cytokine kinetics and no significant expression of anti-inflammatory cytokines.