Effect of the nitrification inhibitors dicyandiamide, nitrapyrin and thiourea onNitrosomonas europaea
Effect of the nitrification inhibitors dicyandiamide, nitrapyrin and thiourea onNitrosomonas europaea
复制标题
DOI：
10.1007/bf01054805
复制
发表时间：
1990-02
期刊：
影响因子：
--
通讯作者：
B. Zacherl;A. Amberger
中科院分区：
文献类型：
--
作者：
B. Zacherl;A. Amberger
在实验室条件下，研究了双氰胺（DCD）、三氯甲基吡啶（N-Serve，NS）和硫脲（TU）对欧洲亚硝化单胞菌生长和代谢的影响。纯培养物的生长被10 ppm NS和0.5 ppm TU完全抑制; 300 ppm DCD的抑制率为83%。亚硝化单胞菌细胞悬液的氨氧化和呼吸作用分别降低了93%（10 ppm NS）、95%（0.5 ppm TU）和73%（300 ppm DCD），而羟胺氧化还原酶不受高浓度抑制剂（200 ppm DCD、100 ppm NS、100 ppm TU）的影响。细胞色素氧化酶活性增加了10%与200 ppm的DCD，不受100 pm TU，和抑制52%与100 ppm的NS。因此，测试的抑制剂之间的作用方式不同。双氰胺的唯一抑菌作用是通过生长抑制的可逆性来证明的。
Effects of the inhibitors dicyandiamide (DCD), nitrapyrin (N-Serve, NS), and thiourea (TU) on growth and metabolism ofNitrosomonas europaeawere tested under laboratory conditions. Growth of a pure culture was completely suppressed by 10 ppm NS and 0.5 ppm TU; inhibition by 300 ppm DCD was 83%. Ammonia oxidation and respiration of Nitrosomonas cell suspensions were reduced by 93% (10 ppm NS), 95% (0.5 ppm TU), and 73% (300 ppm DCD).Hydroxylamine oxidoreductase was not affected by high concentrations of inhibitors (200 ppm DCD, 100 ppm NS, 100 ppm TU). Cytochrome oxidase activity was increased by 10% with 200 ppm DCD, not affected by 100 pm TU, and inhibited by 52% with 100 ppm NS.The modes of action thus differed among the tested inhibitors. The merely bacteriostatic effect of dicyandiamide was demonstrated by the reversibility of growth inhibition.