The expression of inducible nitric oxide synthase in the human fallopian tube during the menstrual cycle and in ectopic pregnancy
The expression of inducible nitric oxide synthase in the human fallopian tube during the menstrual cycle and in ectopic pregnancy
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DOI：
10.1016/j.fertnstert.2009.04.020
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发表时间：
2010-08-01
影响因子：
6.7
通讯作者：
Ledger, William
中科院分区：
文献类型：
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作者：
Al-Azemi, Majedah;Refaat, Bassem;Ledger, William
目的：探讨月经周期期间输卵管 (FT) 中诱导型一氧化氮合酶 (iNOS) 的产生，以及宫外孕 FT 上皮细胞 iNOS 表达是否与健康输卵管不同。设计：前瞻性研究。地点：生殖与发育医学学术单位。患者：月经周期不同阶段的输卵管 (n = 12)，宫外孕 FT (n = 15)，并收集假妊娠妇女 (n = 6) 的 FT。 干预措施：在假妊娠组中，患者在子宫切除术前几天注射 hCG。对样品进行免疫组织化学染色和定量逆转录聚合酶链式反应。主要结果指标：比较不同组之间 iNOS 蛋白和信使 RNA 的表达。结果：这是第一份关于月经周期期间人类输卵管产生 iNOS 周期性的报告。异位妊娠组iNOS表达强度高于假孕组（P < 0.05）。结论：输卵管iNOS表达的周期性提示其参与受精和早期胚胎发育。通过增加 iNOS 产生病理性产生一氧化氮可能会减少输卵管纤毛跳动和平滑肌收缩，从而影响胚胎运输，从而可能导致宫外孕。 （Fertil Steril (R) 2010；94：833-40。(c) 2010 年，美国生殖医学会。）
Objective: To investigate the production of inducible nitric oxide synthase (iNOS) in the fallopian tube (FT) during the menstrual cycle and whether epithelia from FTs bearing an ectopic pregnancy differ from healthy tubes in iNOS expression.Design: Prospective study.Setting: Academic unit of reproductive and developmental medicine.Patient(s): Fallopian tubes from the different stages of the menstrual cycle (n = 12), FTs bearing an ectopic pregnancy (n = 15), and FTs from pseudopregnant women (n = 6) were collected.Intervention(s): In the pseudopregnant group, patients were injected with hCG in the days leading up to hysterectomy. Samples were processed for immunohistochemistry staining and quantitative reverse transcriptase polymerase chain reaction.Main Outcome Measure(s): To compare iNOS protein and messenger RNA expression between the different groups.Result(s): This is the first report on cyclicity in iNOS production by human fallopian tube during the menstrual cycle. The intensity of expression of iNOS was higher in the ectopic pregnancy group compared with the pseudopregnant group (P < 0.05).Conclusion(s): The cyclicity in iNOS expression by the tube suggests its involvement in fertilization and early embryonic development. Pathologic generation of nitric oxide through increase iNOS production may decrease tubal ciliary beats and smooth muscle contractions and thus affect embryo transport, which may consequently result in ectopic pregnancy. (Fertil Steril (R) 2010; 94: 833-40. (c) 2010 by American Society for Reproductive Medicine.)