Four virally determined nuclear antigens are expressed in Epstein-Barr virus-transformed cells.
Four virally determined nuclear antigens are expressed in Epstein-Barr virus-transformed cells.
复制标题
四种病毒确定的核抗原在 Epstein-Barr 病毒转化细胞中表达。
DOI：
10.1073/pnas.83.5.1499
复制
发表时间：
1986
影响因子：
11.1
通讯作者：
Klein,G
中科院分区：
文献类型：
--
作者：
Kallin,B;Dillner,J;Ernberg,I;Ehlin-Henriksson,B;Rosén,A;Henle,W;Henle,G;Klein,G
用健康献血者血清进行免疫印迹试验，研究EB病毒(EBV)相关B细胞生长转化相关抗原的表达。在EB病毒携带细胞系和B淋巴细胞感染转化株B95-8亚株后早期检测到4种抗原。在未感染的细胞中没有发现它们，也不能用缺乏EBV抗原抗体的血清来证明它们。这四种抗原都是核的。在不同的细胞系中，它们的大小各不相同。用16份EBV抗体阳性血清中的6份检测到两个高分子量的抗原，分别为140-160 kDa和150-180 kDa。这些蛋白似乎在抗原性上彼此无关，也与EBNAs 1和2无关，被命名为EBNAs 3和4。与EBNAs 1和EBNAs 2一样，它们在体外与双链和单链DNA结合。
The expression of Epstein-Barr virus (EBV)-determined antigens associated with growth-transformation of B cells was studied by immunoblotting with human sera from healthy donors. Four antigens were detected in EBV-carrying cell lines and in B lymphocytes early after infection with the transforming B95-8 substrain of virus. They were not found in uninfected cells, nor could they be demonstrated with sera lacking antibodies to EBV antigens. All four antigens were nuclear. Each of them varied in size in the different cell lines. The two antigens with the lowest molecular weight were identified as EBV-determined nuclear antigens (EBNAs) 1 and 2. The two high molecular weight antigens (140-160 kDa and 150-180 kDa, respectively) were detected with 6 of 16 EBV antibody-positive sera. These proteins appeared to be antigenically unrelated to each other and to EBNAs 1 and 2 and were designated EBNAs 3 and 4. Like EBNAs 1 and 2, they bound to double- and single-stranded DNA in vitro.