INVIVO RELEASE OF LUTEINIZING-HORMONE RELEASING HORMONE INCREASES WITH PUBERTY IN THE FEMALE RHESUS-MONKEY
INVIVO RELEASE OF LUTEINIZING-HORMONE RELEASING HORMONE INCREASES WITH PUBERTY IN THE FEMALE RHESUS-MONKEY
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DOI：
10.1210/endo-125-1-92
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发表时间：
1989-07-01
期刊：
影响因子：
4.8
通讯作者：
TERASAWA, E
中科院分区：
文献类型：
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作者：
WATANABE, G;TERASAWA, E
为了确定LHRH释放与青春期相关的调节机制，我们使用推拉灌注技术测量了有意识的青春期前和青春期周围雌性猴子的柄-正中隆起LHRH的偶发性释放。将推拉套管插入茎正中隆起后，通过推拉套管注入改良的Krebs-Ringer磷酸盐缓冲液，并在200 .mu处通过拉管收集灌注液。l / 10分钟。用RIA法测定灌注液中的LHRH。每只动物分别在早上（0600-1200小时；0600小时开灯）和晚上（1800-2400小时；1800小时关灯）进行两次6小时采样。分析青春期前LHRH的释放模式(15.7 +-。0.7月龄；的意思。+ -。SEM, n = 6)青春期早期(月经前：26.1 .+-。1.0个月，n = 7)，青春期中期(40.0 +-。1.4个月，n = 6只猴子。结果表明：1)各年龄组LHRH释放具有脉动性。在6只青春期前动物中，有5只LHRH释放为小脉冲（振幅< 2.5 pg/ml），而在所有青春期前动物中，LHRH释放为小脉冲和大脉冲（振幅> 2.5 pg/ml）的混合。2)发育期平均LHRH释放量显著增加（P < 0.02），尤其是在夜间。青春期早、中期组LHRH平均释放量高于青春期前组（上午P < 0.05，晚上P < 0.01）。青春期中期组夜间平均释放量较青春期早期组进一步增加（P < 0.05）。3) LHRH脉幅值也随发育而增加（P < 0.01）。早、中期组脉搏幅值高于青春期前组（早晨P < 0.05，晚上P < 0.02）。从青春期早期到青春期中期，夜间的幅度进一步增加（P < 0.05）。4)发育期基础LHRH释放量增加（P < 0.01）。青春期早期和中期组的傍晚值均高于青春期前组（P < 0.05）。5)脉搏间期随发育而缩短（P < 0.001）。早、中期组脉搏间隔均短于青春期前组（早晨P < 0.01，晚上P < 0.025）。而青春期中期组与青春期早期组的脉搏间隔时间差异无统计学意义。6)青春期中期组夜间平均LHRH和波幅值均显著高于早晨（P < 0.01）。在其他两个年龄组中未观察到昼夜节律波动。这些结果表明：1)在月经初潮前的青春期前期，搏动性LHRH释放增加，同时脉冲频率和脉冲幅值升高；2) LHRH输出仅在青春期中期随脉冲振幅的升高而进一步增加；3)青春期中期夜间LHRH分泌量增加。因此，调控机制的发育变化导致搏动性LHRH释放增加可能是雌性猴子青春期开始的关键因素。
To determine the regulatory mechanism of the LHRH release associated with puberty, episodic release of LHRH from the stalk-median eminence was measured using a push-pull perfusion technique in conscious prepubertal and peripubertal female monkeys. After insertion of a push-pull cannula into the stalk-median eminence, a modified Krebs-Ringer phosphate buffer solution was infused through the push-cannula, and perfusates were collected through the pull-cannula at 200 .mu.l/10 min. LHRH in perfusates was determined by RIA. Two 6-h sampling sessions, in the morning (0600-1200 h; lights on 0600 h) and in the evening (1800-2400 h; lights off 1800 h) were performed in each animal. LHRH release patterns were analyzed in prepubertal (15.7 .+-. 0.7 months of age; mean .+-. SEM, n = 6) early pubertal (premenarcheal: 26.1 .+-. 1.0 months, n = 7), and midpubertal (40.0 .+-. 1.4 months, n = 6) monkeys. Results were as follows: 1) LHRH release was pulsatile in all age groups. While LHRH release in five of six prepubertal animals consisted of small (amplitude < 2.5 pg/ml) pulses, in all peripubertal animals LHRH release was a mixture of small and large (amplitude > 2.5 pg/ml) pulses. 2) There was a significant developmental increase in mean LHRH release (P < 0.02), and this was particularly apparent in the evening. Mean LHRH release in the early and midpubertal groups was higher than that in the prepubertal group (P < 0.05 for morning and P < 0.01 for evening). The mean release in the evening of the midpubertal group further increased over that of the early pubertal group (P < 0.05). 3) Similarly, LHRH pulse amplitude increased developmentally (P < 0.01). Pulse amplitudes in early and midpubertal groups were higher than those in the prepubertal group (P < 0.05 for morning and P < 0.02 for evening). Again the amplitude in the evening further increased from the early pubertal to the midpubertal period (P < 0.05). 4) There was also a developmental increase in basal LHRH release (P < 0.01). The evening values in the early pubertal and midpubertal groups were higher than those in the prepubertal group (P < 0.05). 5) The interpulse interval decreased developmentally (P < 0.001). Interpulse intervals in early and midpubertal groups were shorter than those in the prepubertal group (P < 0.01 for morning and P < 0.025 for evening). However, the interpulse intervals of the midpubertal group were not different from those of early pubertal group. 6) In the midpubertal group evening values of both mean LHRH and amplitude were significantly higher (P < 0.01) than morning values. No circadian fluctuations were observed in the other two age groups. These results suggest that 1) an increase in pulsatile LHRH release with a concomitant elevation in the pulse frequency and pulse amplitude occurred during the early pubertal stage before menarche; 2) LHRH output further increased with an elevation in only the pulse amplitude during the midpubertal stage; and 3) a nocturnal increase in LHRH output occurred during the midpubertal stage. Therefore, developmental changes in a regulatory mechanism resulting in the increased release of pulsatile LHRH may be a key factor responsible for the onset of puberty in female monkeys.