Carbon Nanotubes Deposited on Titanium Implant for Osteoblast Attachment
Carbon Nanotubes Deposited on Titanium Implant for Osteoblast Attachment
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DOI：
10.1166/jbns.2007.003
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发表时间：
2007-06
期刊：
影响因子：
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通讯作者：
N. Aoki;A. Yokoyama;Y. Nodasaka;T. Akasaka;M. Uo;Y. Sato;K. Tohji;F. Watari
中科院分区：
文献类型：
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作者：
N. Aoki;A. Yokoyama;Y. Nodasaka;T. Akasaka;M. Uo;Y. Sato;K. Tohji;F. Watari
种植体表面性能的改善是种植体成功骨结合的关键。本研究尝试将碳纳米管(CNTs)应用于生物医学，并研究其对体外细胞形态的影响。采用电沉积的方法在钛表面涂覆碳纳米管以改善种植体表面，并进行成骨细胞(骨形成细胞)的体外培养。将成骨样细胞(SaOS2)与电沉积碳纳米管(TiCNT)复合，在钛表面培养3d。用扫描电子显微镜(SEM)研究了钛碳纳米管的表面结构和基片上的细胞形态。在细胞培养过程中，电沉积碳纳米管的纤维结构没有改变，碳纳米管没有与钛分离。与聚苯乙烯培养皿上的纺锤形细胞相比，碳纳米管上的细胞呈扁平状，向四面八方分布，与钛上的细胞相似。大量的丝状足孔向碳纳米管网状结构的内侧延伸。这表明碳纳米管具有良好的生物相容性，可以作为细胞黏附蛋白吸附在钛表面的粘结剂进行表面处理。
The improvement of surface properties is very important for successful osseointegration of dental implants. This study attempted to apply carbon nanotubes (CNTs) for biomedical use and investigated the effect of CNTs on cell morphology in vitro. CNT coating was done on Ti by electrodeposition to improve the implant surface and in vitro culture was performed for osteoblastic cells (bone-forming cells). Osteoblast-like cells (SaOS2) were cultured on Ti with electrodeposited CNTs (Ti-CNT) for 3 days. The surface structure of Ti-CNT and the morphology of cells on the substrate were investigated using scanning electron microscopy (SEM). The fibrous structure of electrodeposited CNTs did not change and CNTs did not separate from Ti during cell culture. As compared with the spindle-shape on a polystyrene culture dish, the cells on CNTs were flat and spread out in all directions, similar to those on Ti. Numerous filopodia extended toward the inside of the meshwork of CNTs. This suggests that CNTs have excellent biocompatibility and can be used for surface treatment as a binder for cell-adhesive protein adsorption on the Ti surface.