Valois, a component of the nuage and pole plasm, is involved in assembly of these structures, and binds to Tudor and the methyltransferase Capsuleen
Valois, a component of the nuage and pole plasm, is involved in assembly of these structures, and binds to Tudor and the methyltransferase Capsuleen
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DOI：
10.1242/dev.01809
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发表时间：
2005-05-01
期刊：
影响因子：
4.6
通讯作者：
Mechler, BM
中科院分区：
文献类型：
--
作者：
Anne, J;Mechler, BM
利用酵母双杂交系统中的Capsuleen（Csul）甲基转移酶作为诱饵，我们鉴定了一种新的果蝇蛋白，该蛋白含有多个WD重复序列，由valois（vsl）基因编码，该基因在极浆功能中起作用。Vls与人MEP 50同源，其与PRMT 5甲基转移酶-Csul的人同源物形成复合物。Vls主要定位于滋养细胞的胞浆和卵母细胞的极浆。此外，vls是Oskar的合成和/或稳定性以及Tudor（Tud）在卵母细胞的nuage和后极的定位所必需的。此外，我们表明，Vls和一个片段的Tud直接相互作用的结合试验。由于PMRT 5/MEP 50复合物参与核糖核蛋白复合物的组装，我们推测V1 s复合物可能在组装保育细胞中的nuage和卵母细胞中的极性颗粒中发挥类似的功能。
Using the Capsuleen (Csul) methyltransferase as bait in the yeast two-hybrid system, we have identified a novel Drosophila protein containing multiple WD repeats and encoded by the valois (vsl) gene, which acts in pole plasm function. Vls is homologous to human MEP50, which forms a complex with the PRMT5 methyltransferase-the human homologue of Csul. We found that Vls localizes to the nuage in the nurse cells and to the pole plasm in the oocyte. Moreover vls is required for the synthesis and/or stability of Oskar and the localization of Tudor (Tud) in both the nuage and at the posterior pole of the oocyte. Furthermore, we show that Vls and a fragment of Tud interact directly in binding assay. As the PMRT5/MEP50 complex is involved in ribonucleoprotein complex assembly, we hypothesize that the Vls complex may play a similar function in assembling the nuage in nurse cells and the polar granules in the oocyte.