Comparative cytological responses of lung epithelial and pleural mesothelial cells following in vitro exposure to nanoscale SiO2
Comparative cytological responses of lung epithelial and pleural mesothelial cells following in vitro exposure to nanoscale SiO2
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DOI：
10.1016/j.tiv.2012.09.002
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发表时间：
2013-02-01
影响因子：
3.2
通讯作者：
Sayes, Christie M.
中科院分区：
文献类型：
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作者：
Berg, J. Michael;Romoser, Amelia A.;Sayes, Christie M.
由于独特的表面化学性质和易于使其表面功能化的能力，无定形二氧化硅纳米颗粒正以越来越快的速度被吸收到医药和消费品中。随后，迫切需要了解这些颗粒物与生物系统的相互作用，以试图减轻毒性。肺系统的敏感或耐药细胞类型的鉴定仍然是开发基于纳米颗粒的平台的毒性评估的关键步骤。具体到本研究，检查了A549肺上皮细胞和MeT-5A胸膜间皮瘤细胞系的细胞应答作为检测纳米颗粒诱导的氧化应激的手段。基础表达和细胞抗氧化活性，包括超氧化物歧化酶，过氧化氢酶和谷胱甘肽，进行了检查之前，过氧化氢和类似的30纳米二氧化硅（0.01-100毫克/升）曝光。对氧化剂产生、细胞毒性、GSH耗竭和NRF 2转录因子激活进行了剂量反应观察。结果表明，虽然这两种细胞类型表现出对H2 O2和SiO2诱导的氧化应激和损伤的敏感性，但A549细胞系相对更具弹性。(c)2012爱思唯尔有限公司保留所有权利。
Due to unique surface chemistries and the ability to easily functionalize their surface, amorphous silica nanoparticles are being assimilated into medicinal and consumer products at an increasing rate. Subsequently, there is an emergent need to understand the interactions of these particulates with biological systems in an attempt to mitigate toxicity. The identification of susceptible or resistant cell types of the pulmonary system remains a critical step in the development of toxicity assessments for nanoparticle-based platforms. Specific to this study, the cellular responses of A549 lung epithelial and MeT-5A pleural mesothelial cell lines as a means of detecting nanoparticle-induced oxidative stress were examined. Basal expression and cellular antioxidant activity, including SOD, CAT, and GSH, were examined prior to H2O2 and similar to 30 nm SiO2 (0.01-100 mg/L) exposures. Dose-response observations were made regarding oxidant production, cytotoxicity, GSH depletion and NRF2 transcription factor activation. Results indicated that, while both cell types exhibited susceptibility to H2O2 and SiO2-induced oxidative stress and damage, the A549 cell line was relatively more resilient. (c) 2012 Elsevier Ltd. All rights reserved.