Regulation of onset of female mating and sex pheromone production by juvenile hormone in Drosophila melanogaster
Regulation of onset of female mating and sex pheromone production by juvenile hormone in Drosophila melanogaster
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DOI：
10.1073/pnas.1318119110
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发表时间：
2013-11-05
影响因子：
11.1
通讯作者：
Riddiford, Lynn M.
中科院分区：
文献类型：
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作者：
Bilen, Julide;Atallah, Jade;Riddiford, Lynn M.
保幼激素（JH）协调大多数昆虫雌性生殖成熟的时间。在果蝇中，JH 在交配和卵成熟中发挥作用。然而，人们对与交配相关的分子途径知之甚少。我们对基因上缺乏全体（产生 JH 的腺体）的雌性进行的行为分析表明，与对照雌性相比，它们较少受到雄性的求爱，交配也较晚。在羽化后立即对无乳腺切除的雌性应用 JH 模拟物甲氧普林，挽救了雄性的求爱和交配延迟。我们对 JH 受体无效突变体、Methoprene 耐受 (Met) 和生殖细胞表达 (gce) 的研究表明，Met(27) 雌性中缺乏 Met 会延迟交配的开始，而缺乏 Gce 则影响不大。 Met(27) 的雌性表现出更具吸引力，但行为上不太容易接受交配尝试。 Met 基因组转基因挽救了行为表型，但没有挽救吸引力表型。对女性角质层碳氢化合物谱的分析表明，全体切除导致主要女性特有性信息素（7,11-C27 和 -C29 二烯）产生的延迟以及角质层碳氢化合物混合物的变化。在 Met(27) 无效突变体中，到 48 小时，主要的 C27 二烯相对于野生型大大增加。相比之下，gce(2.5k)无效突变雌性与对照雌性相似，尽管某些角质层碳氢化合物发生了变化。我们的研究结果表明，JH 主要通过 Met 发挥作用，调节雌性性信息素产生和交配的开始时间。
Juvenile hormone (JH) coordinates timing of female reproductive maturation in most insects. In Drosophila melanogaster, JH plays roles in both mating and egg maturation. However, very little is known about the molecular pathways associated with mating. Our behavioral analysis of females genetically lacking the corpora allata, the glands that produce JH, showed that they were courted less by males and mated later than control females. Application of the JH mimic, methoprene, to the allatectomized females just after eclosion rescued both the male courtship and the mating delay. Our studies of the null mutants of the JH receptors, Methoprene tolerant (Met) and germ cell-expressed (gce), showed that lack of Met in Met(27) females delayed the onset of mating, whereas lack of Gce had little effect. The Met(27) females were shown to be more attractive but less behaviorally receptive to copulation attempts. The behavioral but not the attractiveness phenotype was rescued by the Met genomic transgene. Analysis of the female cuticular hydrocarbon profiles showed that corpora allata ablation caused a delay in production of the major female-specific sex pheromones (the 7,11-C27 and -C29 dienes) and a change in the cuticular hydrocarbon blend. In the Met(27) null mutant, by 48 h, the major C27 diene was greatly increased relative to wild type. In contrast, the gce(2.5k) null mutant females were courted similarly to control females despite changes in certain cuticular hydrocarbons. Our findings indicate that JH acts primarily via Met to modulate the timing of onset of female sex pheromone production and mating.