Rapid Identification of Small Interfering Rna that Can Effectively Inhibit the Replication of Multiple Influenza B Virus Strains
Rapid Identification of Small Interfering Rna that Can Effectively Inhibit the Replication of Multiple Influenza B Virus Strains
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DOI：
10.1177/135965350601100411
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发表时间：
2005-05
影响因子：
1.2
通讯作者：
Yongzhen Gao;Lilian Sun;Jie Dong;Xingye Xu;Y. Shu;Mei-hong Chen;L. Yin;Zicai Liang;Q. Jin-Q.
中科院分区：
文献类型：
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作者：
Yongzhen Gao;Lilian Sun;Jie Dong;Xingye Xu;Y. Shu;Mei-hong Chen;L. Yin;Zicai Liang;Q. Jin-Q.
B型流感病毒是导致人类大量发病和死亡的原因，目前的疫苗接种策略和抗病毒药物仅提供有限的保护。在这里，我们报告的小干扰RNA（siRNA）的抑制病毒复制培养细胞以及鸡胚的评价。发现几种靶向病毒保守区域的siRNA（以化学合成或质粒编码的形式）有效地阻断流感B病毒的复制。发现siRNA对几种在遗传内容上显著不同的流感B病毒株（B/Beijing/76/98、B/Beijing/37/99和B/Jiangsu/10/03）提供广泛的保护。发现500 ng siRNA编码质粒或60 nmol合成siRNA的抗病毒效果与3.6 μ g利巴韦林相当。这些结果表明，RNA干扰值得进一步研究的管理流感B病毒感染。
Influenza B virus is a cause of substantial morbidity and mortality in humans and current vaccination strategies and antiviral drugs only provide limited protection. Here, we report the evaluation of small interfering RNA (siRNA) for repression of viral replication in cultured cells as well as in chicken embryos. Several siRNAs targeting conserved regions of the virus (in chemically synthesized or plasmid-encoded forms) were found to effectively block the replication of the influenza B virus. The siRNAs were found to offer broad protection over several strains of influenza B virus (B/Beijing/76/98, B/Beijing/37/99 and B/Jiangsu/10/03) that differ substantially in their genetic content. The antiviral effects of 500 ng siRNA-encoding plasmids or 60 nmoles synthetic siRNA were found to be comparable to that of 3.6 μg ribavirin. These results indicated that RNA interference warrants further study for management of influenza B virus infections.