Effects of an intensive 12-wk training program by elite swimmers on neutrophil oxidative activity.
Effects of an intensive 12-wk training program by elite swimmers on neutrophil oxidative activity.
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优秀游泳运动员为期 12 周的强化训练计划对中性粒细胞氧化活性的影响。
DOI：
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发表时间：
1995
影响因子：
4.1
通讯作者：
M. J. Weidemann
中科院分区：
文献类型：
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作者：
D. Pyne;D. Pyne;D. Pyne;Mark S. Baker;Mark S. Baker;Mark S. Baker;P. Fricker;P. Fricker;P. Fricker;W. A. Mcdonald;W. A. Mcdonald;W. A. Mcdonald;R. Telford;R. Telford;R. Telford;M. J. Weidemann;M. J. Weidemann;M. J. Weidemann
研究了 12 名国家级游泳运动员为期 12 周的强化训练计划对中性粒细胞氧化活性的影响。 11 名久坐（未经训练）的受试者（6 名男性和 5 名女性）充当环境控制者。静息时从肘前静脉采集血样（10ml），并通过标准分离技术分离嗜中性粒细胞。使用荧光探针二氢罗丹明 123 进行体外流式细胞术评估分离的中性粒细胞的氧化爆发活性。双向方差分析（重复测量）显示，在 12 周期间，与久坐对照组相比，优秀游泳运动员的氧化活性较低 (P < 0.05)。对训练中的游泳运动员的细胞进行了分析：重复测量方差分析提供了对体外挑战做出积极反应的细胞数量显着下降（P < 0.05）的证据。尽管有所下降，但游泳者和久坐者自我报告的上呼吸道感染率没有显着差异。这些数据表明：（i）接受强化训练的优秀游泳运动员在休息时的中性粒细胞氧化活性显着低于年龄和性别匹配的久坐个体； (ii) 游泳运动员的氧化活性在剧烈训练期间进一步受到抑制，并且 (iii) 抑制程度似乎不具有临床意义。
The effects of an intensive 12-wk training program by 12 national-level swimmers on neutrophil oxidative activity were studied. Eleven sedentary (untrained) subjects (6 males and 5 females) served as environmental controls. Blood samples (10 ml) were taken at rest from an antecubital vein and neutrophils isolated by standard separation techniques. The oxidative burst activity of isolated neutrophils was assessed with an in vitro flow cytometric assay that used the fluorescent probe dihydrorhodamine 123. Two-way ANOVA (repeated measures) showed that oxidative activity was lower (P < 0.05) in the elite swimmers compared with the sedentary control group across the 12-wk period. Analysis of cells from swimmers in training was made: repeated measures ANOVA provided evidence of a significant decline (P < 0.05) in the number of cells responding positively ito in vitro challenge. Despite this decline, there was no significant difference in self-reported upper respiratory tract infection rate between the swimmers and sedentary individuals. These data show that: (i) elite swimmers undertaking intensive training have a significantly lower neutrophil oxidative activity at rest than do age- and sex-matched sedentary individuals; (ii) aspects of oxidative activity in swimmers are further suppressed during periods of strenuous training, and (iii) the extent of the suppression does not appear to be of clinical significance.