ALFIN-LIKE 6 is involved in root hair elongation during phosphate deficiency in Arabidopsis
ALFIN-LIKE 6 is involved in root hair elongation during phosphate deficiency in Arabidopsis
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DOI：
10.1111/nph.12194
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发表时间：
2013-05-01
期刊：
影响因子：
9.4
通讯作者：
Schmidt, Wolfgang
中科院分区：
文献类型：
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作者：
Chandrika, Nulu Naga Prafulla;Sundaravelpandian, Kalaipandian;Schmidt, Wolfgang
磷（Pi）饥饿诱导植物根毛浓密伸长，增加根系吸收表面积，对植物吸收磷起着重要作用。这些变化背后的分子机制仍不清楚。正向和反向遗传方法被用来确定新的基因参与根毛形成的磷饥饿。在T-DNA插入系的大规模遗传筛选中鉴定了突变体per 2，该突变体在低Pi条件下特异性地具有根毛伸长缺陷。该表型是由同源结构域蛋白ALFIN-LIKE 6（AL 6）中的突变引起的。从筛选基因缺陷突变体，在低Pi培养基上per 2相对于野生型根显示较低的转录丰度，我们确定了四个假定的下游靶标AL 6，即ETC 1，NPC 4，SQD 2和PS2，所有这些都是关键的Pi缺陷植物的根毛伸长。结果进一步表明，AL 6参与控制生长和对Pi饥饿的几个关键反应。我们的研究结果表明，AL 6控制一套基因的转录，根毛伸长在低磷条件下的关键，这表明一个新的生理功能的Alfin基因在拟南芥。
Phosphate (Pi) starvation in plants induces dense and elongated root hairs, which increase the absorptive surface area of the roots and play a critical role in Pi uptake. The molecular mechanism underlying these changes remains unclear. Forward and reverse genetic approaches were employed to identify novel genes involved in root hair formation on Pi starvation. The mutant per2, with defects in root hair elongation specifically under low Pi conditions, was identified in a large-scale genetic screen of T-DNA insertion lines. The phenotype was caused by a mutation in the homeodomain protein ALFIN-LIKE 6 (AL6). From a screen of mutants defective in genes that showed lower transcript abundance in per2 relative to wild-type roots on low Pi medium, we identified four putative downstream targets of AL6, namely ETC1, NPC4, SQD2 and PS2, all of which were critical in root hair elongation of Pi-deficient plants. The results further indicate that AL6 is involved in the control of growth and several key responses to Pi starvation. Our findings demonstrate that AL6 controls the transcription of a suite of genes critical for root hair elongation under low Pi conditions, suggesting a novel physiological function for an Alfin gene in Arabidopsis.