Upconverting nanoparticles as nanotransducers for photodynamic therapy in cancer cells
Upconverting nanoparticles as nanotransducers for photodynamic therapy in cancer cells
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DOI：
10.2217/17435889.3.1.73
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发表时间：
2008-02-01
期刊：
影响因子：
5.5
通讯作者：
Yong, Zhang
中科院分区：
文献类型：
--
作者：
Chatterjee, Dev K.;Yong, Zhang
背景资料：光动力疗法（PDT）涉及通过用高能光激发光敏剂化学物质以从周围溶解的氧产生细胞毒性氧物质来杀死患病细胞。然而，高能量光和疏水光敏剂的不良组织渗透限制了对浅表病变的有效性。上转换磷光体纳米颗粒将低能量辐射转换为更高能量的发射。目的：创建上转换“纳米换能器”，以实现深层组织的PDT。结果如下：在近红外（NIR）激发下产生绿色/红色发射的单分散50 nm PEI/NaYF 4：Yb 3+，Er 3+纳米颗粒靶向人结肠癌细胞上的叶酸受体，并以高信号背景比成像。结果表明，这些微粒在大鼠肌肉注射后可被激发。在NIR激发下，用酞菁锌光敏剂修饰的颗粒释放单线态氧，并且在靶向结合到癌细胞后，导致显著的细胞破坏。结论：这些纳米粒子的潜在临床应用包括深部组织中癌症的成像和PDT。
Background: Photodynamic therapy (PDT) involves killing of diseased cells by excitation of photosensitizer chemicals with high-energy light to produce cytotoxic oxygen species from surrounding dissolved oxygen. However, poor tissue penetration of high-energy light and hydrophobic photosensitizers limits the effectiveness to superficial pathologies. Upconversion phosphor nanoparticles convert low-energy radiation to higher-energy emissions. Aim: To create upconverting 'nanotransducers' to enable PDT in deep tissues. Results: Monodisperse, 50 nm PEI/NaYF4:Yb3+,Er3+ nanoparticles producing green/red emission on near-infrared (NIR) excitation were targeted to folate receptors on human colon cancer cells and imaged with high signal-to-background ratio. It was demonstrated that these particles could be excited after deep intramuscular injection in rats. On NIR excitation, the particles, modified with zinc phthalocyanin photosensitizer, released singlet oxygen and, after targeted binding to cancer cells, resulted in significant cell destruction. Conclusion: Potential clinical use of these nanoparticles includes imaging and PDT of cancer in deep tissues.