Innate Immune Invisible Ultrasmall Gold Nanoparticles-Framework for Synthesis and Evaluation.
Innate Immune Invisible Ultrasmall Gold Nanoparticles-Framework for Synthesis and Evaluation.
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DOI：
10.1021/acsami.1c02834
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发表时间：
2021-05-26
影响因子：
9.5
通讯作者：
Patra HK
中科院分区：
文献类型：
--
作者：
Zhu GH;Azharuddin M;Islam R;Rahmoune H;Deb S;Kanji U;Das J;Osterrieth J;Aulakh P;Ibrahim-Hashi H;Manchanda R;Nilsson PH;Mollnes TE;Bhattacharyya M;Islam MM;Hinkula J;Slater NKH;Patra HK
纳米医学被视为个性化医疗的潜在核心参与者。纳米颗粒的生物相容性问题在过去十年中已基本解决。尽管取得了巨大的进展，但只有不到1%的应用纳米系统可以击中其预期的目标位置，如实体瘤，这仍然是其充分发挥高转化价值的能力和潜力的障碍。因此，获得免疫耐受的、血液相容的和生物友好的纳米颗粒仍然是未满足的需求。纳米制剂从实验室到床边的转化成功涉及对其设计、细胞毒性以外的相容性（如免疫毒性）、血液相容性和免疫介导的破坏/排斥/清除特性的全面评估。在这里，我们报告了一锅工艺工程合成的超小的金纳米粒子（uGNP）适合更好的身体和肾脏清除交付其有效载荷。我们已经获得了低至3 nm的uGNP尺寸，并设计了合成方法，使它们能够精确地尺寸化（几乎是纳米级）。合成的uGNP是生物相容的，并且可以容易地被功能化以携带药物、肽、抗体和其他治疗分子。我们已经用uGNP进行了体外细胞活力测定、免疫毒性测定、炎性细胞因子分析、补体活化研究和凝血研究，以确认它们的安全性。这些可以帮助建立一个长期的安全效益实验框架，以揭示任何设计的纳米颗粒是否是免疫耐受的，并可用作下一代疫苗，化疗药物和治疗诊断剂的有效载荷载体，具有更好的身体清除能力和深层组织渗透。
Nanomedicine is seen as a potential central player in the delivery of personalized medicine. Biocompatibility issues of nanoparticles have largely been resolved over the past decade. Despite their tremendous progress, less than 1% of applied nanosystems can hit their intended target location, such as a solid tumor, and this remains an obstacle to their full ability and potential with a high translational value. Therefore, achieving immune-tolerable, blood-compatible, and biofriendly nanoparticles remains an unmet need. The translational success of nanoformulations from bench to bedside involves a thorough assessment of their design, compatibility beyond cytotoxicity such as immune toxicity, blood compatibility, and immune-mediated destruction/rejection/clearance profile. Here, we report a one-pot process-engineered synthesis of ultrasmall gold nanoparticles (uGNPs) suitable for better body and renal clearance delivery of their payloads. We have obtained uGNP sizes of as low as 3 nm and have engineered the synthesis to allow them to be accurately sized (almost nanometer by nanometer). The synthesized uGNPs are biocompatible and can easily be functionalized to carry drugs, peptides, antibodies, and other therapeutic molecules. We have performed in vitro cell viability assays, immunotoxicity assays, inflammatory cytokine analysis, a complement activation study, and blood coagulation studies with the uGNPs to confirm their safety. These can help to set up a long-term safety-benefit framework of experimentation to reveal whether any designed nanoparticles are immune-tolerable and can be used as payload carriers for next-generation vaccines, chemotherapeutic drugs, and theranostic agents with better body clearance ability and deep tissue penetration.
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影响因子：
17.1
作者：
Huang K;Ma H;Liu J;Huo S;Kumar A;Wei T;Zhang X;Jin S;Gan Y;Wang PC;He S;Zhang X;Liang XJ
通讯作者：
Liang XJ
影响因子：
2.9
作者：
BLUNK, T;HOCHSTRASSER, DF;MULLER, RH
通讯作者：
MULLER, RH
影响因子：
4.7
作者：
Agunloye, Emmanuel;Panariello, Luca;Mazzei, Luca
通讯作者：
Mazzei, Luca
影响因子：
8.6
作者：
Kettemann, Frieder;Birnbaum, Alexander;Polte, Joerg
通讯作者：
Polte, Joerg
粒径和表面电荷对聚合物纳米粒子的细胞摄取和生物分布的影响
影响因子：
14
作者：
He, Chunbai;Hu, Yiping;Yin, Chunhua
通讯作者：
Yin, Chunhua