The Role of Lipopolysaccharide-Induced Extracellular Vesicles in Cardiac Cell Death
The Role of Lipopolysaccharide-Induced Extracellular Vesicles in Cardiac Cell Death
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DOI：
10.3390/biology8040069
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发表时间：
2019-12-01
期刊：
影响因子：
4.2
通讯作者：
Matthews, Qiana L.
中科院分区：
文献类型：
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作者：
Bell, Courtnee' R.;Jones, Leandra B.;Matthews, Qiana L.
外泌体在感染性疾病的进展中起着至关重要的作用，因为外泌体的释放和生物发生受到外部因素如病原性感染的影响。热原可能通过触发炎症、内皮细胞损伤和动脉斑块破裂来帮助疾病的进展，所有这些都可能导致急性冠状动脉疾病，导致心脏组织死亡和心脏事件（CE）的发生。为了更好地理解革兰氏阴性细菌感染对外泌体组成和生物发生的影响，我们检查了用脂多糖（LPS）处理AC 16人心肌细胞后的外泌体特征，所述脂多糖（LPS）作为革兰氏阴性细菌感染的模型系统。使用增加剂量（0、0.1、1或10 μ g）的LPS，我们表明用LPS处理实质上改变了AC 16衍生的外泌体的组成。与未处理组相比，在所有测试浓度的LPS处理下，外来体的相对大小和数量（颗粒/mL）均显著降低。此外，LPS给药降低了与外泌体生物发生相关的外泌体蛋白的表达。相反，我们观察到LPS给药后免疫调节剂增加。LPS对心脏细胞死亡和外泌体组成的影响的这种评估将产生对外泌体在各种生理和病理过程中的重要性的新见解，因为它涉及疾病进展，诊断和治疗。
Exosomes play a crucial role in the progression of infectious diseases, as exosome release and biogenesis are affected by external factors, such as pathogenic infections. Pyrogens may aide in the progression of diseases by triggering inflammation, endothelial cell injury, and arterial plaque rupture, all of which can lead to acute coronary disease, resulting in cardiac tissue death and the onset of a cardiac event (CE). To better understand the effects of Gram-negative bacterial infections on exosome composition and biogenesis, we examined exosome characteristics after treatment of AC16 human cardiomyocytes with lipopolysaccharide (LPS), which served as a model system for Gram-negative bacterial infection. Using increasing doses (0, 0.1, 1, or 10 mu g) of LPS, we showed that treatment with LPS substantially altered the composition of AC16-derived exosomes. Both the relative size and the quantity (particles/mL) of exosomes were decreased significantly at all tested concentrations of LPS treatment compared to the untreated group. In addition, LPS administration reduced the expression of exosomal proteins that are related to exosomal biogenesis. Conversely, we observed an increase in immunomodulators present after LPS administration. This evaluation of the impact of LPS on cardiac cell death and exosome composition will yield new insight into the importance of exosomes in a variety of physiological and pathological processes as it relates to disease progression, diagnosis, and treatment.