Optimized extraction and molecular characterization of polysaccharides from Sophora alopecuroides L. seeds
Optimized extraction and molecular characterization of polysaccharides from Sophora alopecuroides L. seeds
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苦豆子多糖的优化提取及分子表征
DOI：
10.1016/j.ijbiomac.2015.10.002
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发表时间：
2016-01-01
影响因子：
8.2
通讯作者：
Wu, Jinhong
中科院分区：
文献类型：
--
作者：
Guo, Rui;Cao, Nannan;Wu, Jinhong
苦豆子粗多糖提取工艺的优化。结果表明：提取时间为4.15 h，提取温度为70.68 ℃，水/籽比为50.99：1 ml/g。在此临界点处，CSAP的得率和相对粘度最高，分别为10.88%和1.2952，其糖含量为78.40%，蛋白质含量为6.08%，灰分含量为9.71%，水分含量为7.16%，糖醛酸含量为1.88%。通过逐步醇沉淀进一步获得F15、F25、F35和F60的中性级分。单糖组成分析结果表明，CSAP及其中性组分主要由甘露糖和半乳糖组成FT-IR分析表明，产物的分子量为79.45-97.52%，M/G比值为0.94-1.48，是典型的半乳甘露聚糖，重均分子量、回转半径和流体力学半径的大小顺序相同（F15 > P25 > P35 > F60），而对于F25观察到最高的特性粘度。多重分析，包括Mark-Houwink-Sakurada指数（0.48-0.80），持久长度（1.45-3.93 nm），构象分区（无规卷曲）和幂律指数（B > 1），显示所有中性组分的柔性卷曲。关于粘度，在稀溶液中没有F25的聚集体（K "0.60）或线团重叠（“主曲线”）。F25的表面活性较弱可能与其特殊的化学结构和蛋白质部分紧密相连有关。(C)2015 Elsevier B. V.版权所有。
Optimized extraction of crude polysaccharides from Sophora alopecuroides L. seeds (CSAP) was firstly investigated, and the results were extraction time 4.15 h, extraction temperature 70.68 degrees C and water/seed ratio 50.99:1 ml/g. At this proximal point, highest yield (10.88%) and relative viscosity (1.2952) were obtained for CSAP, which contained 78.40% sugar, 6.08% protein, 9.71% ash, 7.16% moisture and 1.88% uronic acid. Neutral fractions of F15, F25, F35 and F60 were further obtained via stepwise alcohol precipitation. Results of monosaccharide composition indicated that CSAP and its neutral fractions mainly consisted of mannose and galactose (79.45-97.52%) with M/G ratios of 0.94-1.48, typical for galactomannans, which were demonstrated by FT-IR. Weight-average molecular weight, radius of gyration and hydrodynamic radius were in the same order (F15 > P25 > P35 > F60), whereas the highest intrinsic viscosity was observed for F25. Multiple analyses, including Mark-Houwink-Sakurada exponent (0.48-0.80), persistence length (1.45-3.93 nm), conformation zoning (random coil) and power-law exponent (b > 1), showed flexible coils for all neutral fractions. Concerning viscometry, there were no aggregates (K '' 0.60) or coil overlaps ("master curve") of F25 in dilute solutions. Relatively weak surface activity of F25 was probably related to its particular chemical structure and proteinaceous moieties closely conjoined. (C) 2015 Elsevier B.V. All rights reserved.