ASSESSMENT OF TOXICITY OF RIVER WATER AND EFFLUENTS BY THE BIOLUMINESCENCE ASSAY USING PHOTOBACTERIUM-PHOSPHOREUM
ASSESSMENT OF TOXICITY OF RIVER WATER AND EFFLUENTS BY THE BIOLUMINESCENCE ASSAY USING PHOTOBACTERIUM-PHOSPHOREUM
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DOI：
10.1016/0043-1354(94)00293-g
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发表时间：
1995-05-01
期刊：
影响因子：
12.8
通讯作者：
CHORDI, A
中科院分区：
文献类型：
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作者：
FERNANDEZ, A;TEJEDOR, C;CHORDI, A
使用发光细菌生物测定法(LUMISTOX系统)评估了Tormes河水流经萨拉曼卡市期间水质的演变。为此，在有关路段研究了所有排放到托尔梅斯河(共22个)的毒性，以及这些排放对河道的影响。由于在快速流动的河流中，低浓度污染或强烈稀释的化合物造成的毒性可能无法被该系统检测到，因此使用XAD-2树脂的有机微污染物浓缩技术被用于检测所研究路段的不同程度的毒性。所分析的所有排放物或多或少都证明对发光菌是有毒的；每种排放物对河流的影响被发现是其EC50和流速(Equitox/m(3))的函数。随着河流接收到不同的排放，河流水的毒性增加(发光抑制(%H15)，抑制发光20%的稀释系数(G120)，以及导致发光在孵化时间15分钟内减少50%的浓缩值(EC50))，从第一个样本(研究排放之前)到最后一个样本(研究放电后)，其中有效浓度50值最大(EC50=2.9)。
The toxicity bioassay employing Photobacterium phosphoreum (LUMISTOX system) was used to evaluate the evolution of the quality of the River Tormes water during its passage through the city of Salamanca. To do so the toxicity of all the discharges into the river Tormes (22 total) were studied in the stretch in question, together with the impact of such discharges on the river course. Since pollution at low concentrations or the toxicity due to compounds that are strongly diluted in a fast flowing river may go undetected by the system,a technique of organic microcontaminant concentration using XAD-2 resins was used to detect different degrees of toxicity along the stretch studied. To a greater or lesser extent, all the discharges analyzed proved to be toxic for the photobacteria; the impact to the river caused by each was found to be a function of its EC50 and flow rate (Equitox/m(3)). The toxicity of the water of the river increases (the inhibition of luminescence (%H15), the dilution factor that inhibits luminescence by 20% (G120) and the concentration values leading to a 50% decrease in the emission of light (EC50) for an incubation time of 15 min, increase) as the river receives the different discharges, from the first sample (before the discharges studied), where the water concentrate has a low effective concentration 50 value (EC50 = 1.3) until the last sample (after the discharges studied), where the effective concentration 50 value is maximum (EC50 = 2.9).