High-Level Congruence of Myrionecta rubra Prey and Dinophysis Species Plastid Identities as Revealed by Genetic Analyses of Isolates from Japanese Coastal Waters
High-Level Congruence of Myrionecta rubra Prey and Dinophysis Species Plastid Identities as Revealed by Genetic Analyses of Isolates from Japanese Coastal Waters
复制标题
DOI：
10.1128/aem.02566-09
复制
发表时间：
2010-05-01
影响因子：
4.4
通讯作者：
Kamiyama, Takashi
中科院分区：
文献类型：
--
作者：
Nishitani, Goh;Nagai, Satoshi;Kamiyama, Takashi
本研究分析了在日本沿海海域采集的天然肉豆杉（Myrionecta rubra）细胞中保留的隐殖核形态18S rRNA基因序列，以及在天然肉豆杉（Dinophysis）细胞中保留的质体16S rRNA基因和psbA基因序列。从134个黄斑鼠细胞中获得715个核形态序列，从71个恐龙细胞中获得564个质体16S rRNA基因和355个psbA序列。几乎所有的序列都与日本沿海的黄鳝相同，说明日本沿海的黄鳝优先摄取黄鳝。其余序列与嗜冷双孢菌（Geminigera cryophila）和尖孢菌（Teleaulax acuta）亲缘关系密切。有趣的是，37个不同于T. amphioxeia的质体16S rRNA基因序列与在格陵兰海发现的D. acuminata细胞中发现的序列相同，这些序列来自于尖锐藻和褐藻细胞，这表明一种广泛分布但未知的隐生物种也被尖锐藻捕食并随后被尖锐藻隔离。为了验证从红斑田鼠和二裂田鼠细胞中分离到的隐生田鼠的分子鉴定的可靠性，本文还对从海水中分离到的隐生田鼠的核形态和质体基因进行了分析。在19株分离株中，分别鉴定出16株和3株为amphioxeia和acuta，种内序列无差异。在日本沿海水域，amphioxeia可能是M. rubra的主要猎物来源。一种未知的隐生植物可以作为额外的来源，这取决于地点和季节。
We analyzed cryptophyte nucleomorph 18S rRNA gene sequences retained in natural Myrionecta rubra cells and plastid 16S rRNA gene and psbA sequences retained in natural cells of several Dinophysis species collected from Japanese coastal waters. A total of 715 nucleomorph sequences obtained from 134 M. rubra cells and 564 plastid 16S rRNA gene and 355 psbA sequences from 71 Dinophysis cells were determined. Almost all sequences in M. rubra and Dinophysis spp. were identical to those of Teleaulax amphioxeia, suggesting that M. rubra in Japanese coastal waters preferentially ingest T. amphioxeia. The remaining sequences were closely related to those of Geminigera cryophila and Teleaulax acuta. Interestingly, 37 plastid 16S rRNA gene sequences, which were different from T. amphioxeia and amplified from Dinophysis acuminata and Dinophysis norvegica cells, were identical to the sequence of a D. acuminata cell found in the Greenland Sea, suggesting that a widely distributed and unknown cryptophyte species is also preyed upon by M. rubra and subsequently sequestered by Dinophysis. To confirm the reliability of molecular identification of the cryptophyte Teleaulax species detected from M. rubra and Dinophysis cells, the nucleomorph and plastid genes of Teleaulax species isolated from seawaters were also analyzed. Of 19 isolates, 16 and 3 clonal strains were identified as T. amphioxeia and T. acuta, respectively, and no sequence variation was confirmed within species. T. amphioxeia is probably the primary source of prey for M. rubra in Japanese coastal waters. An unknown cryptophyte may serve as an additional source, depending on localities and seasons.