The proprotein convertase amontillado (amon) is required during Drosophila pupal development
The proprotein convertase amontillado (amon) is required during Drosophila pupal development
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DOI：
10.1016/j.ydbio.2009.06.021
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发表时间：
2009-09-01
影响因子：
2.7
通讯作者：
Bender, Michael
中科院分区：
文献类型：
--
作者：
Rayburn, Lowell Y. M.;Rhea, Jeanne;Bender, Michael
控制许多发育过程的多肽激素是通过枯草杆菌素样原蛋白转换酶(SPC)家族对不活跃的前体分子进行内蛋白分解而产生的。我们先前发现了果蝇amontillado(Amon)基因的突变，该基因是脊椎动物神经内分泌特异性前激素转换酶2(PC2)基因的同源物，并表明amon在胚胎发生、早期幼虫发育和幼虫蜕皮过程中是必需的。在这里，我们去。在后期发育阶段，使用有条件的救援系统，发现在蛹的发育过程中，头部外翻、腿和翼盘伸展以及腹部分化都需要内阿蒙。免疫定位实验表明，Amon蛋白在中枢神经系统细胞亚群中表达，但不与已知的诱导蜕皮行为的多肽激素共定位，提示新的调节肽参与了这一过程。Amon蛋白在神经细胞中表达，神经细胞分布在环腺的突起和心体，环腺是一个内分泌器官，是许多关键荷尔蒙信号的释放场所。在amon突变背景下使用GAL4/UAS系统在这些细胞类型的子集中表达amon可以部分挽救幼虫的蜕皮和生长。我们的结果表明，amon是蛹发育所必需的，并确定了amon功能足以修复amon突变体所显示的发育进程和生长缺陷的神经细胞类型的子集。研究结果与一种模型相一致，该模型认为氨基蛋白质作用于蛋白质水解性处理一套不同的多肽荷尔蒙，以协调幼虫和幼虫的生长和发育。(C)2009 Elsevier Inc.保留所有权利。
Peptide hormones governing many developmental processes are generated via endoproteolysis of inactive precursor molecules by a family of subtilisin-like proprotein convertases (SPCs). We previously identified mutations in the Drosophila amontillado (amon) gene, a homolog of the vertebrate neuroendocrine-specific Prohormone Convertase 2 (PC2) gene, and showed that amon is required during embryogenesis, early larval development, and larval molting. Here, we de. ne amon requirements during later developmental stages using a conditional rescue system and find that amon is required during pupal development for head eversion, leg and wing disc extension, and abdominal differentiation. Immuno-localization experiments show that amon protein is expressed in a subset of central nervous system cells but does not co-localize with peptide hormones known to elicit molting behavior, suggesting the involvement of novel regulatory peptides in this process. The amon protein is expressed in neuronal cells that innervate the corpus allatum and corpora cardiaca of the ring gland, an endocrine organ which is the release site for many key hormonal signals. Expression of amon in a subset of these cell types using the GAL4/UAS system in an amon mutant background partially rescues larval molting and growth. Our results show that amon is required for pupal development and identify a subset of neuronal cell types in which amon function is sufficient to rescue developmental progression and growth defects shown by amon mutants. The results are consistent with a model that the amon protein acts to proteolytically process a diverse suite of peptide hormones that coordinate larval and pupal growth and development. (C) 2009 Elsevier Inc. All rights reserved.