Petunia bZIP transcription factor PhbZIP3 is involved in floral volatile benzenoid/phenylpropanoid biosynthesis through regulating the expression of PhPAL2 and PhBSMT
Petunia bZIP transcription factor PhbZIP3 is involved in floral volatile benzenoid/phenylpropanoid biosynthesis through regulating the expression of PhPAL2 and PhBSMT
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DOI：
10.1016/j.scienta.2023.112719
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发表时间：
2023-11-29
影响因子：
4.3
通讯作者：
Zhang，Chao
中科院分区：
文献类型：
--
作者：
Fu，Jianxin;Yu，Guo;Zhang，Chao
花挥发性苯/苯丙化合物（FVBPs）是植物必需的次生代谢产物，在植物生殖发育中起着关键作用。然而，其生物合成途径的转录调控知识仍然有限。虽然碱性亮氨酸拉链（bZIP）家族广泛参与植物次生代谢的调节，但尚未报道其调节FVBP的生物合成。本研究旨在筛选牵牛花FVBP生物合成转录调控中重要的bZIP转录因子成员。研究人员确定了矮牵牛基因组中bzipgenes的全部序列，并深入研究了它们的系统发育关系、结构特征、表达模式以及与FVBP生物合成相关结构基因的潜在相互作用。结果表明，矮牵牛的77bZIPgenes可分为11个类群。矮牵牛花bzip成员内含子和保守基序的显著数量差异，以及顺式作用元件的多样性，反映了矮牵牛花bzip结构的多样性。根据RNA测序数据，选择了7个调控FVBP生物合成的候选zipgene，因为它们在花开放后表达水平上调。基于qPCR和亚细胞定位分析，筛选出一个核定位的成员phbzip3。phbzip3主要在花冠中表达，在花期表达量显著增加。双荧光素酶检测结果显示，PhbZIP3可以增强phpal2和phbsmt的表达。酵母单杂交实验的结果进一步证实了PhbZIP3与phpal2和phbsmt启动子之间的相互作用。综上所述，这些结果表明PhbZIP3在牵牛花FVBP生物合成的调控机制中发挥了关键作用。
Floral volatile benzenoid/phenylpropanoid compounds (FVBPs) are essential plant secondary metabolites and play a pivotal role in plant reproduction and development. However, the knowledge of transcriptional regulation of its biosynthesis pathway remains limited. Although extensively implicated in the regulation of plant secondary metabolisms, the basic leucine zipper (bZIP) family has not been reported to regulate FVBP biosynthesis. This study aimed to screen out important bZIP transcription factor members in the transcriptional regulation of FVBP biosynthesis in petunia. The entirety ofbZIPgenes throughout the petunia genome was ascertained and in-depth investigations into their phylogenetic relationships, structural characteristics, expression patterns, and potential interactions with structural genes related to FVBP biosynthesis were conducted. Outcomes showed that 77bZIPgenes of petunia could be categorized into 11 groups. The significant quantitative differences of introns and conserved motifs among the petuniabZIPmembers, along with the variety ofcis-acting elements, reflected the structure diversity of petunia bZIPs. Seven candidatebZIPgenes for regulating FVBP biosynthesis were selected because of their up-regulated expression level after flower opening according to RNA sequencing data. Based on the qPCR and the subcellular localization analysis, one nuclear-localized member,PhbZIP3was screened out.PhbZIP3was mainly expressed in the corolla, and its expression level increased significantly during the anthesis period. Dual luciferase assay revealed that PhbZIP3 could enhance the expression ofPhPAL2andPhBSMT. The findings from the yeast one-hybrid assay further corroborated the interaction between PhbZIP3 and the promoters ofPhPAL2andPhBSMT. Taken together, these results suggest that PhbZIP3 play a pivotal role in the regulatory mechanism of FVBP biosynthesis in petunia.