Vibrio cholerae 01 can assume a chlorine-resistant rugose survival form that is virulent for humans
Vibrio cholerae 01 can assume a chlorine-resistant rugose survival form that is virulent for humans
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DOI：
10.1093/infdis/174.6.1364
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发表时间：
1996-12-01
影响因子：
6.4
通讯作者：
Johnson, JA
中科院分区：
文献类型：
--
作者：
Morris, JG;Sztein, MB;Johnson, JA
霍乱弧菌可以转变为与促进细胞聚集的无定形外多糖的表达相关的“皱纹”菌落形态。流式细胞术研究表明，皱纹培养物中高达3%的颗粒代表>5个细菌细胞的聚集体。我们的测试菌株的皱纹变体显示出对氯杀死的抗性，活细胞在2 mg/L游离氯中持续>30 min;菌株还显示出对补体介导的血清杀菌活性的杀死的抗性。6名志愿者喂食10（6）cfu霍乱弧菌O 1 El Tor Inaba N16961的皱纹变体，出现典型的霍乱症状，平均粪便量为2.2 L（范围1.4-4.3）。从受感染志愿者粪便中回收的分离株保留了皱纹表型。这些数据表明，皱纹菌株导致人类疾病。这些菌株在霍乱流行病学中的作用仍有待确定。
Vibrio cholerae can shift to a ''rugose'' colonial morphology associated with expression of an amorphous exopolysaccharide that promotes cell aggregation. Flow cytometric studies indicated that up to 3% of particles in rugose cultures represented aggregates of >5 bacterial cells. Rugose variants of our test strains displayed resistance to killing by chlorine, with viable cells persisting for >30 min in 2 mg/L free chlorine; strains also showed resistance to killing by complement-mediated serum bactericidal activity. Six volunteers fed 10(6) cfu of a rugose variant of V. cholerae O1 El Tor Inaba N16961 developed symptoms typical of cholera, with a mean diarrheal stool volume of 2,2 L (range, 1.4-4.3). Isolates recovered from the stool of infected volunteers retained the rugose phenotype. The data suggest that rugose strains cause human disease. The role of these strains in the epidemiology of cholera remains to be determined.