A novel dual-layer forward osmosis membrane for protein enrichment and concentration
A novel dual-layer forward osmosis membrane for protein enrichment and concentration
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DOI：
10.1016/j.seppur.2009.08.002
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发表时间：
2009-10
影响因子：
8.6
通讯作者：
Qian Yang;K. Wang;T. Chung
中科院分区：
文献类型：
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作者：
Qian Yang;K. Wang;T. Chung
我们展示了双层聚苯并咪唑-聚醚砜/聚乙烯吡咯烷酮（PBI-PES /PVP）中空纤维纳滤（NF）膜在正向渗透（FO）过程中的富集和浓缩的前景，而不会使感兴趣的成分变性。采用共挤技术制备的双层中空纤维膜具有10μm左右的超薄选择性表皮、完全开孔的水通道和微孔海绵状支撑结构。自充电PBI选择性皮肤的平均孔径半径为0.4nm，孔径分布明显。实验结果表明，新研制的双层中空纤维纳滤膜作为FO膜使用时，溶菌酶富集通量高，蛋白质污染少。此外，该双层膜对Mg2+的高二价盐抑制率约为90%，确保了富集的溶菌酶产物具有高纯度且不发生变化和变性。
We have demonstrated the prospect of dual-layer polybenzimidazole-polyethersulfone/polyvinylpyrrolidone (PBI–PES/PVP) hollow fiber nanofiltration (NF) membranes in the forward osmosis (FO) process for the enrichment and concentration of pharmaceutical products without denaturing the component of interests. The dual-layer hollow fiber membrane via coextrusion technology has an ultra-thin selective skin around 10μm, fully open-cell water channels underneath and a microporous sponge-like support structure. The self-charged PBI selective skin has an average pore radius at 0.4nm with a sharp pore size distribution. Experimental results show that the newly developed dual-layer hollow fiber nanofiltration membrane can achieve a high throughput for lysozyme enrichment and less protein fouling when using it as a FO membrane. In addition, the high divalent salt rejection towards Mg2+at around 90% of this dual-layer membrane ensures the enriched lysozyme product with high purity and without change and denaturing.