Cloning and characterization of boron transporters in Brassica napus
Cloning and characterization of boron transporters in Brassica napus
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DOI：
10.1007/s11033-011-0930-z
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发表时间：
2011-06
影响因子：
2.8
通讯作者：
Jinhua Sun;Lei Shi;Chunyu Zhang;Fangsen Xu
中科院分区：
文献类型：
--
作者：
Jinhua Sun;Lei Shi;Chunyu Zhang;Fangsen Xu
Six full-length cDNA encoding boron transporters (BOR) were isolated fromBrassica napus(AACC) by rapid amplification of cDNA ends (RACE). The phylogenic analysis revealed that the sixBORs were the orthologues ofAtBOR1,which formed companying with the triplication and allotetra-ploidization process ofB. napus,and were divided into three groups inB. napus. Each group was comprised of two members, one of which was originated fromBrassica rapa(AA) and the other fromBrassica oleracea(CC). Based on the phylogenetic relationships, the six genes were named asBnBOR1;1a,BnBOR1;1c,BnBOR1;2a,BnBOR1;2c,BnBOR1;3aandBnBOR1;3c, respectively. The deducedBnBOR1s had extensive similarity with other plantBORs, with the identity of 74–96.8% in amino acid sequence. TheBnBOR1;3aandBnBOR1;3cresembledAtBOR1in number and positions of the 11 introns, but the others only have 9 introns. After the gene duplication, there was evidence of purifying selection under a divergent selective pressure. The expression patterns of the sixBnBOR1swere detected by semi-quantitative RT–PCR. TheBnBOR1;3aandBnBOR1;3cshowed a ubiquitous expression in all of the investigated tissues, whereas the other four genes showed similar tissue-specific expression profile. Unlike the non-transcriptional regulation ofAtBOR1, the expression ofBnBOR1;1candBnBOR1;2awere obviously induced by boron deficiency. This study suggested that theBOR1s had undergone a divergent expression pattern in the genome ofB. napusafter that theB. napusdiverged fromArabidopsis thaliana.
