Transcutaneous immunization using common chemicals
Transcutaneous immunization using common chemicals
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DOI：
10.1016/j.jconrel.2009.05.003
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发表时间：
2009-09-01
影响因子：
10.8
通讯作者：
Mitragotri, Samir
中科院分区：
文献类型：
--
作者：
Karande, Pankaj;Arora, Anubhav;Mitragotri, Samir
经皮免疫是疫苗在皮肤上的局部应用，它比针头免疫有几个优点。然而，由于疫苗通过皮肤角质层的运输有限，简单的局部应用疫苗不能产生足够的免疫反应。在这里，我们报告了常见护肤品中使用的化学物质可以增强局部使用的抗原的免疫原性。对600种常用化学品的配方进行了系统的体外筛选，以确定其效力(抗原传递)。随后在体外测试了这些制剂的一个选定的子集的佐剂性(免疫反应的激活)。铅配方在体内测试了它们产生针对局部应用的卵清蛋白的抗体滴度的能力，卵清蛋白是一种模式抗原。铅制剂在产生抗卵清蛋白抗体效价方面明显更有效。我们的结果表明，化学制剂可以成功地用于传递抗原，并且可以通过对单个化学成分的组合筛选来合理地设计这种制剂。(C)2009爱思唯尔B.V.保留所有权利
Transcutaneous immunization, topical application of vaccines on skin, provides several advantages over needle based immunization. However, simple topical application of vaccines does not generate sufficient immune response due to limited transport of vaccines across the stratum corneum of skin. Here we report that chemicals used in common skin products can enhance the immunogenicity of topically applied antigens. Six hundred formulations of commonly used chemicals were screened systematically for their potency (delivery of antigen) in vitro. A selected subset of these formulations was subsequently tested for their adjuvanticity (activation of immune response) in vitro. Lead formulations were tested in vivo for their ability to generate antibody titers against topically applied ovalbumin, a model antigen. Lead formulations were significantly more effective in generating anti-ovalbumin IgG titers. Our results demonstrate that chemical formulations can be successfully used to deliver antigens and that such formulations can be rationally designed by combinatorial screening of individual chemical components. (C) 2009 Elsevier B.V. All rights reserved