EX-VIVO INDUCTION OF TNF-ALPHA AND IL-6 MESSENGER-RNA IN BOVINE WHOLE-BLOOD BY MYCOBACTERIUM-PARATUBERCULOSIS AND MYCOBACTERIAL CELL-WALL COMPONENTS
EX-VIVO INDUCTION OF TNF-ALPHA AND IL-6 MESSENGER-RNA IN BOVINE WHOLE-BLOOD BY MYCOBACTERIUM-PARATUBERCULOSIS AND MYCOBACTERIAL CELL-WALL COMPONENTS
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DOI：
10.1006/mpat.1995.0041
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发表时间：
1995-07-01
影响因子：
3.8
通讯作者：
CZUPRYNSKI, CJ
中科院分区：
文献类型：
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作者：
ADAMS, JL;CZUPRYNSKI, CJ
约翰氏病是牛和其他反刍动物的慢性肠炎，具有世界性的经济重要性。细胞因子肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）与其他疾病综合征中的肉芽肿形成和消耗有关。这些细胞因子在Johne病的发展和进展中的潜在作用尚未研究。使用逆转录聚合酶链反应（RT-PCR）和特异性牛寡核苷酸细胞因子引物和探针牛TNF-α和IL-6，我们检查了这些炎症细胞因子在健康牛全血中的离体表达。牛全血与副结核分枝杆菌或其脂阿拉伯甘露聚糖（LAM）短暂孵育后，细胞因子mRNA水平增加。胞壁酰二肽（MDP）和大肠杆菌LPS也刺激TNF-α和IL-6 mRNA的表达。几株M.对副结核杆菌进行了测试，发现它们具有类似的刺激TNF-α和IL-6 mRNA表达的能力。几种密切相关的鸟分枝杆菌菌株和不相关的腐殖酸分枝杆菌草分枝杆菌刺激TNF-α和IL-6 mRNA表达的能力稍低。(C)1995年学术出版社
Johne's disease is a chronic enteritis of cattle and other ruminant species that is of worldwide economic importance. The cytokines tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) have been associated with granuloma formation and wasting in other disease syndromes. The potential role of these cytokines in the development and progression of Johne's disease has not been investigated. Using reverse transcriptase polymerase chain reaction (RT-PCR) and specific bovine oligonucleotide cytokine primers and probes for bovine TNF-alpha and IL-6, we examined the ex vivo expression of mRNA for these inflammatory cytokines in whole blood from healthy cattle. Cytokine mRNA levels increased after a brief incubation of bovine whole blood with Mycobacterium paratuberculosis or its lipoarabinomannan (LAM). Muramyl dipeptide (MDP) and Escherichia coli LPS also stimulated TNF-alpha and IL-6 mRNA expression. Several strains of M. paratuberculosis were tested and found to have similar abilities to stimulate TNF-alpha and IL-6 mRNA expression. Several strains of the closely related Mycobacterium avium, and the unrelated saprophyte, Mycobacterium phlei, had somewhat less ability to stimulate TNF-alpha and lL-6 mRNA expression. (C) 1995 Academic Press Limited