INDUCTION OF MORPHOLOGICAL AND FUNCTIONAL-DIFFERENTIATION OF HUMAN PROMYELOCYTIC LEUKEMIA-CELLS (HL-60) BY COMPOUNDS WHICH INDUCE DIFFERENTIATION OF MURINE LEUKEMIA-CELLS
INDUCTION OF MORPHOLOGICAL AND FUNCTIONAL-DIFFERENTIATION OF HUMAN PROMYELOCYTIC LEUKEMIA-CELLS (HL-60) BY COMPOUNDS WHICH INDUCE DIFFERENTIATION OF MURINE LEUKEMIA-CELLS
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DOI：
10.1002/ijc.2910250208
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发表时间：
1980-01-01
影响因子：
6.4
通讯作者：
GALLO, RC
中科院分区：
文献类型：
--
作者：
COLLINS, SJ;BODNER, A;GALLO, RC
多种化合物对促进某些小鼠白血病细胞系（Friend红白血病细胞和小鼠髓性白血病细胞）体外分化的能力进行了测试，以诱导HL-60细胞的分化，HL-60细胞是一种能够在体外最终分化为功能成熟的粒细胞的人类早幼粒细胞白血病细胞系。极性平面化合物包括六亚甲基双乙酰胺（HMBA）、某些嘌呤（特别是次黄嘌呤）和放线菌素d诱导HL-60的形态和功能分化（通过减少NBT染料的能力来评估）。相比之下，血红素、瓦巴因、前列腺素[PG]E1、x射线照射、地塞米松等抗白血病化疗药物对HL-60细胞的分化作用很小，甚至没有明显的诱导作用。这些结果与先前对小鼠白血病细胞的观察结果表明，人类HL-60细胞与某些小鼠白血病细胞具有共同的细胞靶位点，可以诱导极平面化合物、次黄嘌呤和放线菌素- d的作用。相比之下，hemin、ouabain和PGE1可能对小鼠红白血病细胞具有特异性，而x射线照射和化疗药物可诱导小鼠白血病细胞两种类型（红系和髓系）的分化，但对HL-60细胞的影响很小。
Various compounds active in promoting in vitro differentiation of certain murine leukemia cell lines (Friend erythroleukemia cells and mouse myeloid leukemia cells) were tested for their capacity to induce differentiation of HL-60 cells, a human promyelocytic leukemia cell line capable of terminally differentiating in vitro to functionally mature granulocytes. Polar planar compounds including hexamethylene bisacetamide (HMBA), certain purines (particularly hypoxanthine) and actinomycin-D induced morphological and functional (as assessed by the capacity to reduce NBT dye) differentiation of HL-60. In contrast, hemin, ouabain, prostaglandin[PG]E1, X-irradiation, dexamethasone and some other anti-leukemic chemotherapeutic agents induced little if any significant differentiation of HL-60 cells. These results, together with previous observations with murine leukemia cells, suggest that the human HL-60 cells share common cellular target sites for the inducing action of polar planar compounds, hypoxanthine and actinomycin-D with some murine leukemic cells. In contrast, hemin, ouabain and PGE1 may be specific for mouse erythroleukemia cells, while X-irradiation and chemotherapeutic agents induce differentiation of both types (erythroid and myeloid) of mouse leukemic cells, but have little effect on HL-60 cells.