TYROSINE PHOSPHATASE CD45 IS REQUIRED FOR T-CELL ANTIGEN RECEPTOR AND CD2-MEDIATED ACTIVATION OF A PROTEIN TYROSINE KINASE AND INTERLEUKIN-2 PRODUCTION
TYROSINE PHOSPHATASE CD45 IS REQUIRED FOR T-CELL ANTIGEN RECEPTOR AND CD2-MEDIATED ACTIVATION OF A PROTEIN TYROSINE KINASE AND INTERLEUKIN-2 PRODUCTION
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DOI：
10.1073/pnas.88.6.2037
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发表时间：
1991-03-01
影响因子：
11.1
通讯作者：
WEISS, A
中科院分区：
文献类型：
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作者：
KORETZKY, GA;PICUS, J;WEISS, A
CD45是一种造血细胞特异性表面抗原，最近被证明是一种蛋白酪氨酸磷酸酶。CD45的表达对于T细胞抗原受体与磷脂酰肌醇第二信使通路的偶联以及抗原介导的T淋巴细胞增殖至关重要。在本报告中，我们描述了人类t细胞白血病系Jurkat的cd45缺陷突变体。CD45的表达是激活t细胞受体相关酪氨酸激酶以及磷脂酰肌醇途径所必需的。此外，通过辅助分子CD2刺激T淋巴细胞需要CD45的表达。cd45缺陷细胞中的突变特异性地损害t细胞受体和CD2的信号转导，因为通过另一个辅助分子CD28的激活事件未受到损害。
CD45, a hematopoietic cell-specific surface antigen, has recently been shown to be a protein tyrosine phosphatase. Expression of CD45 is essential for the T-cell antigen receptor to couple with the phosphatidylinositol second messenger pathway and for antigen-mediated proliferation of T lymphocytes. In this report we describe a CD45-deficient mutant of the human T-cell leukemia line Jurkat. CD45 expression is required for the activation of a T-cell receptor-associated tyrosine kinase as well as the phosphatidylinositol pathway. Additionally, stimulation of T lymphocytes by way of the accessory molecule CD2 requires the expression of CD45. The mutation in the CD45-deficient cell specifically impairs signal transduction by the T-cell receptor and CD2 because activation events by way of another accessory molecule, CD28, are unimpaired.