Effect of transplanting suprachiasmatic nuclei from donors of different ages into completely SCN lesioned hamsters.
Effect of transplanting suprachiasmatic nuclei from donors of different ages into completely SCN lesioned hamsters.
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DOI：
10.1155/np.1993.257
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发表时间：
1993-10
期刊：
影响因子：
--
通讯作者：
Menaker M
中科院分区：
文献类型：
--
作者：
Kaufman CM;Menaker M
视交叉上核（SCN）是哺乳动物的主要昼夜节律起搏器。Ralph和同事们最近为此提供了新的证据，他们将SCN移植到具有不同基因决定的周期的金黄仓鼠之间，并产生了具有捐赠者特征周期的转轮活动的昼夜节律。我们已经扩展了这些研究，以评估可用于移植的供体组织的年龄范围。从胚胎第11天到出生后第12天的仓鼠SCN可以作为功能性移植物，以恢复运动性SCN损伤动物的节律性。SCN移植和心律发作之间的时间不依赖于供体的年龄。含有血管活性肠肽（VIP）免疫反应细胞的补片的存在是移植成功的良好指标，而其缺乏与缺乏移植效果相关。在此期间，可以收获SCN组织用于移植的18天跨度应该扩展该技术可以投入的用途。我们的研究结果还表明，这将是有利的，以检查的年龄范围的神经组织，可用于其他移植模型。
The suprachiasmatic nucleus (SCN) is the primary circadian pacemaker in mammals. Ralph and colleagues/14/provided recent new evidence for this by transplanting SCNs between golden hamsters with different genetically determined periods and producing circadian rhythmsof running wheel activity with periods characteristic of the donor. We have extended these studies in order to evaluate the age range of donor tissue that can be used for transplantation. SCN of hamsters from embryonic day 11 through postnatal day 12 can serve as functional grafts to restore rhythmicity to arrhythmic SCN lesioned animals. The time between SCN transplantation and onset of rhythmicity does not depend on the age of the donor. The presence of patches containing vasoactive intestinal peptide (VIP) immunoreactive cells is a good indicator of graft success, while its absence is correlated with a lack of transplant effect. The 18 day span during which SCN tissue can be harvested for transplantation should expand the uses to which this technique can be put. Our results also suggest that it would be advantageous to examine the age range of neural tissue that ca’n be used in other transplantation models.