Rapid substrate-induced charge movements of the GABA transporter GAT1
Rapid substrate-induced charge movements of the GABA transporter GAT1
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DOI：
10.1529/biophysj.105.061002
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发表时间：
2005-07-01
影响因子：
3.4
通讯作者：
Grewer, C
中科院分区：
文献类型：
--
作者：
Bicho, A;Grewer, C
GABA转运体GAT1通过将GABA摄取与两个钠离子和一个氯离子的共同转运偶联，将神经递质GABA从突触间隙中移除。这项工作的目的是研究GAT1在GABA浓度跃升后的个别反应步骤。在HEK293细胞中瞬时表达了GAT1，并将膜片钳技术与笼式GABA的激光脉冲光解相结合，研究了GAT1的稳态前动力学。
The GABA transporter GAT1 removes the neurotransmitter GABA from the synaptic cleft by coupling of GABA uptake to the co-transport of two sodium ions and one chloride ion. The aim of this work was to investigate the individual reaction steps of GAT1 after a GABA concentration jump. GAT1 was transiently expressed in HEK293 cells and its pre-steady-state kinetics were studied by combining the patch-clamp technique with the laser-pulse photolysis of caged GABA, which allowed us to generate GABA concentration jumps within