PECT1, a rate‐limiting enzyme in phosphatidylethanolamine biosynthesis, is involved in the regulation of stomatal movement in Arabidopsis
PECT1, a rate‐limiting enzyme in phosphatidylethanolamine biosynthesis, is involved in the regulation of stomatal movement in Arabidopsis
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PECT1 是磷脂酰乙醇胺生物合成的限速酶，参与拟南芥气孔运动的调节
DOI：
10.1111/tpj.16245
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Iba Koh
中科院分区：
文献类型：
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作者：
Negi Juntaro;Obata Tomoki;Nishimura Sakura;Song Boseok;Yamagaki Sho;Ono Yuhei;Okabe Makoto;Hoshino Natsumi;Fukatsu Kohei;Tabata Ryo;Yamaguchi Katsushi;Shigenobu Shuji;Yamada Masashi;Hasebe Mitsuyasu;Sawa Shinichiro;Kinoshita Toshinori;Nishida Ikuo;Iba Koh
通过叶片热成像筛选分离出显示对CO2的气孔反应受损的Ancillaropsismutant，cdi 4。突变基因PECT 1编码CTP：磷酸乙醇胺胞苷酰转移酶。Thecdi 4表现出磷脂酰乙醇胺水平的降低和光诱导的气孔开放以及低CO2诱导的气孔开放的缺陷。我们建立了在保卫细胞中特异性抑制PECT 1的RNAi细胞系。这些品系的气孔对低CO2浓度或光照的反应受损。真菌毒素fusicoccin（FC）通过磷酸化激活保卫细胞质膜H+-ATPases，促进气孔开放，而AHA 1在保卫细胞中高表达，FC激活AHA 1可诱导气孔开放。ecdi 4和PECT 1 RNAi株系表现出对FC的气孔开放反应降低。然而，与野生型相似，cdi 4在FC处理后维持气孔H+-ATP酶的磷酸化和活化的正常水平。此外，di 4显示出正常的GFP-AHA 1融合蛋白定位和正常水平的AHA 1转录本。基于这些结果，我们讨论了PECT 1如何以独立于H+-ATP酶激活的下游方式调节保卫细胞中CO2和光诱导的气孔运动。[2023年5月15日首次在线发布后添加的更正：本版本中修订了第三句。
AnArabidopsismutant displaying impaired stomatal responses to CO2,cdi4, was isolated by a leaf thermal imaging screening. The mutated genePECT1encodes CTP:phosphorylethanolamine cytidylyltransferase. Thecdi4exhibited a decrease in phosphatidylethanolamine levels and a defect in light‐induced stomatal opening as well as low‐CO2‐induced stomatal opening. We created RNAi lines in whichPECT1was specifically repressed in guard cells. These lines are impaired in their stomatal responses to low‐CO2concentrations or light. Fungal toxin fusicoccin (FC) promotes stomatal opening by activating plasma membrane H+‐ATPases in guard cells via phosphorylation.ArabidopsisH+‐ATPase1 (AHA1) has been reported to be highly expressed in guard cells, and its activation by FC induces stomatal opening. Thecdi4andPECT1RNAi lines displayed a reduced stomatal opening response to FC. However, similar to in the wild‐type,cdi4maintained normal levels of phosphorylation and activation of the stomatal H+‐ATPases after FC treatment. Furthermore, thecdi4displayed normal localization of GFP‐AHA1 fusion protein and normal levels ofAHA1transcripts. Based on these results, we discuss how PECT1 could regulate CO2‐ and light‐induced stomatal movements in guard cells in a manner that is independent and downstream of the activation of H+‐ATPases. [Correction added on 15 May 2023, after first online publication: The third sentence is revised in this version.]