Relationship of fluid and mucin secretion to morphological changes in the perfused rat submandibular gland.
Relationship of fluid and mucin secretion to morphological changes in the perfused rat submandibular gland.
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DOI：
10.1076/ejom.40.4.203.16691
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发表时间：
2002-10-01
期刊：
影响因子：
--
通讯作者：
Riva, A.
中科院分区：
文献类型：
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作者：
Murakami, M.;Yoshimura, K.;Riva, A.
电解质/水的转运和胞吐作用通过胞质Ca 2+浓度的升高而激活，并且通过胞质环AMP的升高而增强。为了将粘液和液体分泌与形态学变化相关联，对大鼠下颌下腺进行血管灌注，并在用不同浓度的卡巴胆碱（CCh）和/或异丙肾上腺素（ISP）刺激期间测量唾液中的液体分泌和N-乙酰半乳糖胺。单刺激1 μ M CCh诱导的N-乙酰半乳糖胺的短暂增加，随后下降到一个较低的水平，在持续的刺激。1 μ M ISP的过载增加了N-乙酰半乳糖胺的分泌到一个更高的持续水平40-50 μ g/g-min。然而，在1 μ M CCh，液体分泌维持在相同的水平，在刺激过程中，甚至过载1 μ M ISP没有显着影响其水平，而除了0.5 μ M ISP的腺体刺激0.1 μ M CCh增加液体分泌。用高分辨扫描电镜（HRSEM）和透射电镜（TEM）对样品进行了形貌观察。不同浓度的CCh和ISP联合作用后，细胞形态发生了明显的变化，反映了细胞的液体分泌和粘蛋白分泌。使用P-31 NMR测量ATP和磷酸肌酸（PCr）的动力学，这表明由于有限的能量供应，在较高水平的CCh刺激下，液体分泌的增强是有限的。
Transport of electrolytes/water and exocytosis are activated by elevation of the cytosolic Ca2+ concentration and are potentiated by elevation of cytosolic cyclic AMP. To correlate mucin and fluid secretion with morphological changes, rat submandibular glands were vascularly perfused and the fluid secretion and N-acetylgalactosamine in the saliva were measured during stimulation with various concentrations of carbachol (CCh) and/or isoproterenol (ISP). Single stimulation with 1 muM CCh induced a transient increase of N-acetyl galactosamine followed by a decline to a low level during sustained stimulation. The overload of 1 muM ISP increased secretion of N-acetyl galactosamine to a higher sustained level of 40-50 mug/g-min. However, at 1 muM CCh, fluid secretion was maintained at the same level during stimulation and even overload of 1 muM ISP did not significantly affect its level, whereas addition of 0.5 muM ISP to the gland stimulated with 0.1 muM CCh increased fluid secretion. Morphological observation was carried out by HRSEM and TEM. Combination of CCh and ISP in different concentrations resulted in distinctive morphological changes which reflect fluid secretion and mucin secretion. The kinetics of ATP and creatine phosphate (PCr) were measured using P-31 NMR, which indicated that the potentiation of fluid secretion is limited under a higher level of CCh stimulation due to a limited energy supply.