Electron transport controls transcription of the thioredoxin gene (trxA) in the cyanobacterium Synechocystis sp. PCC 6803
Electron transport controls transcription of the thioredoxin gene (trxA) in the cyanobacterium Synechocystis sp. PCC 6803
复制标题
电子传输控制蓝藻集胞藻中硫氧还蛋白基因 (trxA) 的转录。
DOI：
10.1023/a:1006472018601
复制
发表时间：
2000
影响因子：
5.1
通讯作者：
F. Florencio
中科院分区：
文献类型：
--
作者：
F. Navarro;E. Martín;F. Florencio
编码兼性异养蓝细菌集胞藻属PCC 6803的不同硫氧还蛋白之一的trxA基因转录为450个核苷酸的单个mRNA。转录物积累在所有标准生长条件下相似，但在将细胞培养物从光照转移到黑暗后强烈降低。在稳态条件下，与中等（10-50 μ E m − 2 s − 1）光强相比，高光强（150-500 μ E m − 2 s − 1）下trxA转录减少。trxA转录物的稳定性在不同的光强度下是相似的，并且在黑暗中也是如此。光合电子传递抑制剂，以及在缺乏光系统II的突变株葡萄糖饥饿，促进trxA转录水平的强烈下降。引物延伸分析表明，trxA是从两个含有−10 TATA盒的近端启动子转录的，与大肠杆菌共有启动子相似。与trxA mRNA不同，硫氧还蛋白蛋白的量在黑暗中不减少，在高光强度下也不减少，表明硫氧还蛋白蛋白非常稳定。我们的研究结果表明，由trxA基因编码的硫氧还蛋白可能主要是在转录水平上，而不是在蛋白质水平上，通过光合作用或葡萄糖代谢产生的电子传递进行调节。
The trxA gene encoding one of the different thioredoxins of the facultative heterotrophic cyanobacterium Synechocystis sp. PCC 6803 is transcribed as a single mRNA of 450 nucleotides. Transcript accumulation is similar in all standard growth conditions but strongly decreases after transferring cell cultures from light to darkness. In steady-state conditions, trxA transcription is reduced at high (150–500 μE m−2 s−1) compared with moderate (10–50 μE m−2 s−1) light intensities. The stability of the trxA transcript was similar at different light intensities, and also in darkness. Photosynthetic electron transport inhibitors, as well as glucose starvation in a mutant strain lacking photosystem II, promote a strong decline in the level of trxA transcript. Primer extension analysis suggests that trxA is transcribed from two proximal promoters containing a −10 TATA box similar to the Escherichia coli consensus promoters. Unlike the trxA mRNA, the amount of thioredoxin protein was not reduced in the dark, neither at high light intensities, indicating that thioredoxin protein is very stable. Our results indicate that the thioredoxin encoded by the trxA gene is likely to be primarily regulated at the transcriptional level, rather than at the protein level, by the electron transport generated photosynthetically or from glucose metabolism.
硫氧还蛋白对于光合作用生长至关重要。
DOI：
--
发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Muller,EG;Buchanan,BB
通讯作者：
Buchanan,BB
DOI：
10.1016/s0021-9258(18)31570-9
发表时间：
1991-05
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Eric G D Muller
通讯作者：
Eric G D Muller
影响因子：
56.9
作者：
Kim, JM;Mayfield, SP
通讯作者：
Mayfield, SP