CLONING AND CHARACTERIZATION OF A POTENTIALLY PROTECTIVE ANTIGEN IN LYMPHATIC FILARIASIS
CLONING AND CHARACTERIZATION OF A POTENTIALLY PROTECTIVE ANTIGEN IN LYMPHATIC FILARIASIS
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DOI：
10.1073/pnas.85.10.3604
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发表时间：
1988-05-01
影响因子：
11.1
通讯作者：
KAZURA, JW
中科院分区：
文献类型：
--
作者：
NILSEN, TW;MARONEY, PA;KAZURA, JW
为了促进保护性丝虫抗原的生化研究，从马来丝虫成体mRNA构建了.lambda.gt11 cDNA文库，并用识别大约25、42、60和112kDa的有限丝虫抗原的兔血清进行筛选。 .apprxeq 的抗原。 25 和 .apprxeq。先前已证明 60 kDa 可诱导小鼠微丝丝虫血症的清除增强。通过免疫反应检测到的154个碱基对的克隆用于通过杂交分离出1.8kb的几乎全长的cDNA克隆。核苷酸序列分析表明该克隆源自编码 63 kDa 抗原的 mRNA。使用针对.apprxeq的部分纯化制剂产生的抗血清，在免疫印迹实验中识别出含有37kDa的大肠杆菌TrpE蛋白（邻氨基苯甲酸合酶）和55kDa的克隆蛋白的融合多肽。 60-kDa 保护性丝虫抗原。这些数据将克隆的抗原与淋巴丝虫病中的潜在保护性抗原联系起来。
To facilitate biochemical studies of protective filarial antigens, a .lambda.gt11 cDNA library was constructed from Brugia malayi adult mRNA and screened with rabbit sera that recognizes a limited set of filarial antigens of approximately 25, 42, 60, and 112 kDa. Antigens of .apprxeq. 25 and .apprxeq. 60 kDa have been shown previously to induce enhanced clearance of microfilaremia in mice. A 154-base pair clone detected by immunological reactivity was used to isolate by hybridization a nearly full-length cDNA clone of 1.8 kilobases. Nucleotide-sequence analysis indicated that this clone was derived from a mRNA encoding a 63-kDa antigen. A fusion polypeptide containing 37 kDa of the Escherichia coli TrpE protein (anthranilate synthase) and 55 kDa of the cloned protein was recognized in immunoblot experiments with antisera raised against a partially purified preparation of the .apprxeq. 60-kDa protective filarial antigen. Thee data relate the cloned antigen to a potentially protective antigen in lymphatic filariasis.